Ing. Kristýna Slámová, PhD.

Position:
Senior Scientist
Email:
slamova@biomed.cas.cz
Phone:
(+420) 296 442 766
Address:
Laboratory of Biotransformation
Institute of Microbiology of the Czech Academy of Sciences
Vídeňská 1083
Praha 4 – Krč
142 20
Czech Republic

Education

  • Institute of Chemical Technology, Prague – Ing. (General and Applied Biochemistry; 2007)
  • Institute of Chemical Technology, Prague – PhD. (Microbiology; 2012)
  • Since 2005 member of the Laboratory of Biotransformation

Research Orientation

  • Application of glycosidases, mainly β-N-acetylhexosami­nidases, in the biocatalytic synthesis of novel functionalized oligosaccharides
  • 3D Structure and biotransformation abilities of β-N-acetylhexosami­nidases from filamentous fungi
  • Production of human, bacterial and fungal β-N-acetylhexosami­nidases in microbial expression systems
  • Functional mutants of fungal β-N-acetylhexosami­nidases
  • Search for selective inhibitors of β-N-acetylhexosami­nidases and β-N-acetylglucosa­minidases

Experience

  • 2006 – ICRM-CNR, Milano, Italy – Dr. Sergio Riva (4 weeks)
  • 2009 – RWTH Aachen, Germany – Prof. Lothar Elling (5 weeks)

Projects

  • New synthetic approaches using mutant and wild-type β-N-acetylhexosami­nidase from Talaromyces flavus. (project 13–06818P, Czech Science Foundation; 2013–2016)
  • Galectins as biomarkers of colorectal cancer (project LTC 19035, Ministry of Education, Youth and Sports, 2019-2022)
  • Evolution of fungal transglycosidases for the preparation of antimicrobial chitooligosaccharides applicable in crop protection (project 20-00477S, Czech Science Foundation, 2020-2022)

Member of COST Actions:

  • TRANSCOLONCAN CA17118 (Identifying biomarkers through translational research for prevention and stratification of colorectal cancer)
  • INNOGLY CA18103 (Innovations with glycans: new frontiers from synthesis to new biological targets)

Selected Recent Publications

  • Patrick Weber, Zuzana Mészáros, Pavla Bojarová, Manuel Ebner, Roland Fischer, Vladimír Křen, Natalia Kulik, Philipp Müller, Miluše Vlachová, Kristýna Slámová, Arnold E. Stütz, Martin Thonhofer, Ana Torvisco, Tanja M. Wrodnigg, Andreas Wolfsgruber: Highly functionalized diaminocyclopentanes: A new route to potent and selective inhibitors of human O-GlcNAcase. Bioorganic Chemistry, 140, November 2023, 106819: 1-14 (2023). 
  • Kristýna Slámová, Jakub Červený, Zuzana Mészáros, Tereza Friede, David Vrbata, Vladimír Křen, Pavla Bojarová: Oligosaccharide Ligands of Galectin-4 and Its Subunits: Multivalency Scores Highly. Molecules, 28 (10), 4039: 1-15 (2023). 
  • Patrick Weber, Zuzana Mészáros, Denis Jagečić, Valentina Hribljan, Dinko Mitrečić, Pavla Bojarová, Kristýna Slámová, Jiří Vrba, Natalia Kulik, Vladimír Křen, Arnold E. Stütz: Diaminocyclopentane-derived O-GlcNAcase inhibitors for combating tau hyperphosphorylation in Alzheimer’s disease. Chemical Communications, 58 (63), 8838-8841 (2022).
  • Katerina Brodsky, Kristýna Káňová, Dorota Konvalinková, Kristýna Slámová, Helena Pelantová, Kateřina Valentová, Pavla Bojarová, Vladimír Křen, Lucie Petrásková: Bacterial Aryl Sulfotransferases in Selective and Sustainable Sulfation of Biologically Active Compounds using Novel Sulfate Donors. ChemSusChem, 15 (18), e202201253: 1-9 (2022). 
  • Pavlína Nekvasilová, Kulik Natalia, Michael Kotík, Lucie Petrásková, Kristýna Slámová, Vladimír Křen, Pavla Bojarová: Mutation Hotspot for Changing the Substrate Specificity of β-N-Acetylhexosaminidase: A Library of GlcNAcases. International Journal of Molecular Sciences, 23 (20), 12456: 1-17 (2022). 
  • Zuzana Mészáros, Lucie Petrásková, Natalia Kulik, Helena Pelantová, Pavla Bojarová, Vladimír Křen, Kristýna Slámová: Hypertransglycosylating Variants of the GH20 β-N-Acetylhexosaminidase for the Synthesis of Chitooligomers. Advanced Synthesis & Catalysis, 364 (12), 2009-2022 (2022). 
  • Pavlína Nekvasilová, Michaela Hovorková, Zuzana Mészáros, Lucie Petrásková, Helena Pelantová, Vladimír Křen, Kristýna Slámová, Pavla Bojarová: Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides. International Journal of Molecular Sciences, 23 (8), 4106:1-13 (2022). 
  • Pingitore Valeria, Martínez-Bailén Macarena, Carmona T. Ana, Mészáros Zuzana, Kulik Natalia, Slámová Kristýna, Křen Vladimír, Bojarová Pavla, Robina Inmaculada, Moreno-Vargas J. Antonio: Discovery of human hexosaminidase inhibitors by in situ screening of a library of mono- and divalent pyrrolidine iminosugars. Bioorganic Chemistry, 120, 105650:1-12 (2022). 
  • Mariann Kiss, István Timári, Teréz Barna, Zuzana Mészáros, Kristýna Slámová, Pavla Bojarová, Vladimír Křen, Joseph M. Hayes, László Somsák: 2-Acetamido-2-deoxy-D-glucono-1,5-lactone Sulfonylhydrazones: Synthesis and Evaluation as Inhibitors of Human OGA and HexB Enzymes. International Journal of Molecular Sciences, 23 (3), 1037 1-19 (2022). 
  • Sedlář Antonín, Trávníčková Martina, Matějka Roman, Pražák Šimon, Mészáros Zuzana, Bojarová Pavla, Bačáková Lucie, Křen Vladimír, Slámová Kristýna: Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation. International Journal of Molecular Sciences, 22 (4), 1843 1-27 (2021). 
  • Mészáros Zuzana, Nekvasilová Pavlína, Bojarová Pavla, Křen Vladimír, Slámová Kristýna: Advanced glycosidases as ingenious biosynthetic instruments. Biotechnology Advances, 49, 107733, 1-27 (2021). 
  • Antonín Sedlář, Martina Trávníčková, Pavla Bojarová, Miluše Vlachová, Kristýna Slámová, Vladimír Křen, Lucie Bačáková: Interaction between Galectin-3 and Integrins Mediates Cell-Matrix Adhesion in Endothelial Cells and Mesenchymal Stem Cells. International Journal of Molecular Sciences, 22 (10), 5144, 1-26 (2021). 
  • Jansa Jan, Šmilauer Petr, Borovička Jan, Hršelová Hana, Forczek T. Sándor, Slámová Kristýna, Řezanka Tomáš, Rozmoš Martin, Bukovská Petra, Gryndler Milan: Dead Rhizophagus irregularis biomass mysteriously stimulates plant growth. Mycorrhiza, 30 (1), 63-77 (2020). 
  • Jana Kapešová, Lucie Petrásková, Natalia Kulik, Zuzana Straková, Pavla Bojarová, Kristína Markošová, Martin Rebroš, Vladimír Křen, Kristýna Slámová: Transglycosidase activity of glycosynthase-type mutants of a fungal GH20 β-N-acetylhexosaminidase. International Journal of Biological Macromolecules, 161, 1206-1215 (2020). 
  • Pavlína Nekvasilová, Natalia Kulik, Nikola Rychlá, Helena Pelantová, Lucie Petrásková, Zuzana Bosáková, Josef Cvačka, Kristýna Slámová, Vladimír Křen, Pavla Bojarová: How Site-Directed Mutagenesis Boosted Selectivity of a Promiscuous Enzyme. Advanced Synthesis and Catalysis, 362 (19), 4138-4150 (2020). 
  • Bojarová Pavla, Kulik Natalia, Hovorková Michaela, Slámová Kristýna, Pelantová Helena, Křen Vladimír: The β-N-Acetylhexosaminidase in the Synthesis of Bioactive Glycans: Protein and Reaction Engineering. Molecules, 24 (3), 599, 1-14 (2019). 
  • Bojarová Pavla, Kulik Natallia, Slámová Kristýna, Hubálek Martin, Kotík Michael, Cvačka Josef, Pelantová Helena, Křen Vladimír: Selective β-N-acetylhexosaminidase from Aspergillus versicolor—a tool for producing bioactive carbohydrates. Applied Microbiology and Biotechnology, 103 (4), 1737-1753 (2019). 
  • Slámová Kristýna, Kapešová Jana, Valentová Kateřina: „Sweet Flavonoids“: Glycosidase-Catalyzed Modifications. International Journal of Molecular Sciences, 19 (7), 2126 (1-19) (2018). 

 

Last update: May 31, 2023