Ing. Kristýna Slámová, PhD.

Position:
Senior Scientist
Email:
slamova@biomed.cas.cz
Phone:
(+420) 296 442 766
Address:
Laboratory of Biotransformation
Institute of Microbiology of the Czech Academy of Sciences
Vídeňská 1083
Praha 4 – Krč
142 20
Czech Republic

Education

  • Institute of Chemical Technology, Prague – Ing. (General and Applied Biochemistry; 2007)
  • Institute of Chemical Technology, Prague – PhD. (Microbiology; 2012)
  • Since 2005 member of the Laboratory of Biotransformation

Research Orientation

  • Application of glycosidases, mainly β-N-acetylhexosami­nidases, in the biocatalytic synthesis of novel functionalized oligosaccharides
  • 3D Structure and biotransformation abilities of β-N-acetylhexosami­nidases from filamentous fungi
  • Production of human, bacterial and fungal β-N-acetylhexosami­nidases in microbial expression systems
  • Functional mutants of fungal β-N-acetylhexosami­nidases
  • Search for selective inhibitors of β-N-acetylhexosami­nidases and β-N-acetylglucosa­minidases

Experience

  • 2006 – ICRM-CNR, Milano, Italy – Dr. Sergio Riva (4 weeks)
  • 2009 – RWTH Aachen, Germany – Prof. Lothar Elling (5 weeks)

Projects

  • New synthetic approaches using mutant and wild-type β-N-acetylhexosami­nidase from Talaromyces flavus. (project 13–06818P, Czech Science Foundation; 2013–2016)
  • Galectins as biomarkers of colorectal cancer (project LTC 19035, Ministry of Education, Youth and Sports, 2019-2022)
  • Evolution of fungal transglycosidases for the preparation of antimicrobial chitooligosaccharides applicable in crop protection (project 20-00477S, Czech Science Foundation, 2020-2022)

Member of COST Actions:

  • TRANSCOLONCAN CA17118 (Identifying biomarkers through translational research for prevention and stratification of colorectal cancer)
  • INNOGLY CA18103 (Innovations with glycans: new frontiers from synthesis to new biological targets)

Selected Recent Publications

  • Patrick Weber, Zuzana Mészáros, Denis Jagečić, Valentina Hribljan, Dinko Mitrečić, Pavla Bojarová, Kristýna Slámová, Jiří Vrba, Natalia Kulik, Vladimír Křen, Arnold E. Stütz: Diaminocyclopentane-derived O-GlcNAcase inhibitors for combating tau hyperphosphorylation in Alzheimer’s disease. Chemical Communications, 58 (63), 8838-8841 (2022).
  • Katerina Brodsky, Kristýna Káňová, Dorota Konvalinková, Kristýna Slámová, Helena Pelantová, Kateřina Valentová, Pavla Bojarová, Vladimír Křen, Lucie Petrásková: Bacterial Aryl Sulfotransferases in Selective and Sustainable Sulfation of Biologically Active Compounds using Novel Sulfate Donors. ChemSusChem, 15 (18), e202201253: 1-9 (2022)
  • Pavlína Nekvasilová, Kulik Natalia, Michael Kotík, Lucie Petrásková, Kristýna Slámová, Vladimír Křen, Pavla Bojarová: Mutation Hotspot for Changing the Substrate Specificity of β-N-Acetylhexosaminidase: A Library of GlcNAcases. International Journal of Molecular Sciences, 23 (20), 12456: 1-17 (2022)
  • Zuzana Mészáros, Lucie Petrásková, Natalia Kulik, Helena Pelantová, Pavla Bojarová, Vladimír Křen, Kristýna Slámová: Hypertransglycosylating Variants of the GH20 β-N-Acetylhexosaminidase for the Synthesis of Chitooligomers. Advanced Synthesis & Catalysis, 364 (12), 2009-2022 (2022)
  • Pavlína Nekvasilová, Michaela Hovorková, Zuzana Mészáros, Lucie Petrásková, Helena Pelantová, Vladimír Křen, Kristýna Slámová, Pavla Bojarová: Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides. International Journal of Molecular Sciences, 23 (8), 4106:1-13 (2022)
  • Pingitore Valeria, Martínez-Bailén Macarena, Carmona T. Ana, Mészáros Zuzana, Kulik Natalia, Slámová Kristýna, Křen Vladimír, Bojarová Pavla, Robina Inmaculada, Moreno-Vargas J. Antonio: Discovery of human hexosaminidase inhibitors by in situ screening of a library of mono- and divalent pyrrolidine iminosugars. Bioorganic Chemistry, 120, 105650:1-12 (2022)
  • Mariann Kiss, István Timári, Teréz Barna, Zuzana Mészáros, Kristýna Slámová, Pavla Bojarová, Vladimír Křen, Joseph M. Hayes, László Somsák: 2-Acetamido-2-deoxy-D-glucono-1,5-lactone Sulfonylhydrazones: Synthesis and Evaluation as Inhibitors of Human OGA and HexB Enzymes. International Journal of Molecular Sciences, 23 (3), 1037 1-19 (2022)
  • Sedlář Antonín, Trávníčková Martina, Matějka Roman, Pražák Šimon, Mészáros Zuzana, Bojarová Pavla, Bačáková Lucie, Křen Vladimír, Slámová Kristýna: Growth Factors VEGF-A165 and FGF-2 as Multifunctional Biomolecules Governing Cell Adhesion and Proliferation. International Journal of Molecular Sciences, 22 (4), 1843 1-27 (2021)
  • Mészáros Zuzana, Nekvasilová Pavlína, Bojarová Pavla, Křen Vladimír, Slámová Kristýna: Advanced glycosidases as ingenious biosynthetic instruments. Biotechnology Advances, 49, 107733, 1-27 (2021)
  • Antonín Sedlář, Martina Trávníčková, Pavla Bojarová, Miluše Vlachová, Kristýna Slámová, Vladimír Křen, Lucie Bačáková: Interaction between Galectin-3 and Integrins Mediates Cell-Matrix Adhesion in Endothelial Cells and Mesenchymal Stem Cells. International Journal of Molecular Sciences, 22 (10), 5144, 1-26 (2021)
  • Jansa Jan, Šmilauer Petr, Borovička Jan, Hršelová Hana, Forczek T. Sándor, Slámová Kristýna, Řezanka Tomáš, Rozmoš Martin, Bukovská Petra, Gryndler Milan: Dead Rhizophagus irregularis biomass mysteriously stimulates plant growth. Mycorrhiza, 30 (1), 63-77 (2020)
  • Jana Kapešová, Lucie Petrásková, Natalia Kulik, Zuzana Straková, Pavla Bojarová, Kristína Markošová, Martin Rebroš, Vladimír Křen, Kristýna Slámová: Transglycosidase activity of glycosynthase-type mutants of a fungal GH20 β-N-acetylhexosaminidase. International Journal of Biological Macromolecules, 161, 1206-1215 (2020)
  • Pavlína Nekvasilová, Natalia Kulik, Nikola Rychlá, Helena Pelantová, Lucie Petrásková, Zuzana Bosáková, Josef Cvačka, Kristýna Slámová, Vladimír Křen, Pavla Bojarová: How Site-Directed Mutagenesis Boosted Selectivity of a Promiscuous Enzyme. Advanced Synthesis and Catalysis, 362 (19), 4138-4150 (2020)
  • Bojarová Pavla, Kulik Natalia, Hovorková Michaela, Slámová Kristýna, Pelantová Helena, Křen Vladimír: The β-N-Acetylhexosaminidase in the Synthesis of Bioactive Glycans: Protein and Reaction Engineering. Molecules, 24 (3), 599, 1-14 (2019)
  • Bojarová Pavla, Kulik Natallia, Slámová Kristýna, Hubálek Martin, Kotík Michael, Cvačka Josef, Pelantová Helena, Křen Vladimír: Selective β-N-acetylhexosaminidase from Aspergillus versicolor—a tool for producing bioactive carbohydrates. Applied Microbiology and Biotechnology, 103 (4), 1737-1753 (2019)
  • Slámová Kristýna, Kapešová Jana, Valentová Kateřina: „Sweet Flavonoids“: Glycosidase-Catalyzed Modifications. International Journal of Molecular Sciences, 19 (7), 2126 (1-19) (2018)

 

Last update: November 4, 2022