Major Laboratory Equipment

Prominence Shimadzu HPLC-LCMS 2020

Configuration

Two high-pressure solvent delivery units (the LC-20AD). The pumps are configured as high resolution (0.1%) binary- high pressure gradient system. Auto-sampler (SIL-20AC), column oven  (CTO-10A, + 10°C to 85°C), PDA detector (SPD-M20A) offer a very low baseline noise of less than 0.35×10–5 AU, fraction collector (FRC-10A), mass detector (LCMS-2020) is a single quadrupole mass spectrometer with ESI ionization.

Prominence Shimadzu HPLC

Configuration

Two high-pressure solvent delivery units (the LC-20AB), auto-sampler (SIL-20A) column oven (CTO-20A), PDA Detector (SPD-M20A).

 

 

Waters Analytical HPLC

The ternary gradient pump, Waters 600 E Multisolvent Delivery System, provides flows up to 10 ml/min. The system enables manual or automatic injection by Rheodyne injection valve or Waters717plus Autosampler, respectively. The system is equipped with Waters 996 PDA Detector and Waters 2410 Differential Refractometer. The columns available involve various reversed-phase, normal phase, ion exchange and chiral columns.

 

Shimadzu isocratic preparative HPLC

Isocratic preparative HPLC pump LC-8A with mobile phase flow up to 150 ml/min is equipped with manual injection valve Rheodyne. Preparative fraction collector FRC-10A is controlled by operator or by signal from SPD-20A UV detector. Columns available: include normal (silicagel) and RP-18 50×250 mm columns and Asahipack GS-310 multimode GPC column.

 

NGC QuestTM10 Plus Protein Chromatography System

Bio-Rad Laboratories, Inc.

This up-to-date protein chromatography system is suitable for all types of manually performed or automated protein purification techniques, including affinity, ion-exchange, and hydrophobic chromatography as well as high-pressure gel filtration.

Equipment:

NGC F10 Pump module, 10 ml/min, max. 25 MPa
NGC Sample Pump module, 100 ml/min, max. 10 MPa
NGC Mixer module
NGC Sample Inject Valve module
NGC Inlet Valve module
Integrated Touchscreen
Bio-Frac Fraction collector
 

Agilent 6890N Network Gas Chromatograph

Agilent 6890N network GC system is equipped with single FID, single split/splitless capillary with EPC, G2613A 7683 injector-autosampler, Agilent Chemstation with G2070A single GC software.

 

 

ÄKTApurifier™

Manufactured by Amersham Biosciences, Sweden

ÄKTApurifier™ is a versatile protein and peptide purification system compatible with any type of chromatographic columns and solvents. This FPLC can operate at maximum flow rate of 10 ml/min with a maximum pressure of 25 MPa, which is sufficient for preparative protein purification on a milligram scale. Our instrument is equipped with a P-903 pump (2 pump modules A and B, including two pump heads each), Monitor UV-900 (able to detect up to three wavelengths simultaneously), Monitor pH/C-900 (measurement of pH and conductivity, pH electrode), and a Frac-920 fraction collector. The system is connected to a PC and controlled by Unicorn 5.11 software.

This instrument is used for the high performance purification of fungal glycosidases and nitrilases (ion exchange chromatography, hydrophobic interaction chromatography, gel filtration, chromatofocusing, affinity chromatography and reversed phase chromatography).

ÄKTAprime™ plus

Manufactured by Amersham Biosciences, Sweden

ÄKTAprime™ plus is a rather simple and user-friendly FPLC for protein chromatography, employing maximum pressure of 1 MPa. The unit is compact and comprises of a system pump, fraction collector, monitors for UV, conductivity and pH. The system is not PC controlled but together with PrimeView™ software it offers continuous real-time monitoring and evaluation of results.

This system is used for the protein purification steps that do not require high operation pressure (ion exchange chromatography, hydrophobic interaction chromatography, affinity chromatography).

Microtitration plate reader Tecan Sunrise

Manufacturer: Tecan (Austria)

Sunrise is a versatile microplate absorbance reader designed for simple analysis of 96-well plates. Sunrise supports a large variety of absorbance based immunoassay protocols (immunometric methods, such as ELISA assays; competitive and homo- or heterogenous immunoassays), kinetic assays or colorimetric cell toxicity assays. The facility is fully automated and controlled by computer using software „Magellan“. The facility enables the measurement at two different wavelength simultaneously, temperate the plates to desired temperature and shake the plates.

Technical parameters
Wavelength range: 340 – 750 nm
Measurement time: single wavelength: 6 seconds, dual wavelength: 8 seconds
Resolution: 0.001 Abs
Plate temperature: from 5 °C above ambient to 42 °C

PCR Thermocycler Biometra Tpersonal

Manufacturer: Biometra (Germany)

The Biometra Tpersonal thermocycler provides maximum performance on minimum space. Thanks to a new generation of Peltier elements, high heating and cooling rates have been achieved leading thus to short run times. This facility is equipped by a combined block for both 0.2 and 0.5 ml tubes.

Technical parameters
Temperature range: –3 – 99 °C
Maximal heating and cooling rate: 3 °C/s
Control accuracy: +/- 0.1 °C
Lid temperature range: 30 – 99 °C

Incubator shaker IKA KS 4000i control

Manufacturer: IKA (Germany)

Technical parameters
Temperature range: 5 °C above ambient to 80 °C
Temperature accuracy: +/- 0.1 °C
Speed range: 10 – 500 rpm
Dimensions: 580×750×520 mm