Ing. Lucie Petrásková, PhD.

Position:
Senior Scientist
Email:
petraskova@biomed.cas.cz
Phone:
(+420) 296 442 766
Address:
Laboratory of Biotransformation
Institute of Microbiology of the Czech Academy of Sciences
Vídeňská 1083
Praha 4 – Krč
142 20
Czech Republic

Education

  • Institute of Chemical Technology, Prague, Faculty of Food Chemistry and Biochemistry – Applied Biology and Bioengineering – MSc (2000)

PhD Thesis

  • Enzymatic synthesis of glycoconjugates – new substrates and new enzymes

Supervisors

Research Orientation

  • biotransformations, chromatographic separation

Long-term Visits Abroad

  • Istituto di Biocatalisi e Riconoscimento Molecolare, Milano, Italy – in group of Dr. Sergio Riva (4 weeks), 2000.
  • L. Kossuth University, Debrecen, Hungary (1 week), 2001.
  • Institut für Organische Chemie, Universität Hamburg, Germany – in group of Prof. Thiem (4weeks), 2001.
  • FEBS/NATO Summer school – Chemical Probes in Biology, Greece (2 weeks), 2002.
  • Instituto de Química Orgánica by Centro de Investigaciones Biológicas, C. S. I. C., Madrid – in group of Prof. Dr. Jesús Jiménez-Barbero (2 weeks), 2003.
  • CNR-NATO advanced fellowship in group of Dr. Sergio Riva, Milano, Italy, 6 months, 2004–2005.

Publications

Selected publications 

  • Miluše Vlachová, Van Nguyen Tran, Jakub Červený, František Dolníček, Lucie Petrásková, Helena Pelantová, Ondřej Kundrát, Josef Cvačka, Zuzana Bosáková, Vladimír Křen, Pavel Lhoták, Jitka Viktorová, Pavla Bojarová: Galectin-targeting glycocalix[4]arenes can enter the cells†. Chemical Communications, 59, 10404-10407 (2023). 
  • Dorota Konvalinková, František Dolníček, Michaela Hovorková, Jakub Červený, Ondřej Kundrát, Helena Pelantová, Lucie Petrásková, Josef Cvačka, Margarita Faizulina, Beena Varghese, petr Kovaříček, Vladimír Křen, Pavel Lhoták, Pavla Bojarová: Glycocalix[4]arenes and their affinity to a library of galectins: the linker matters. Organic & Biomolecular Chemistry, 21 (6), 1294-1302 (2023). 
  • Vrbata David, Filipová Marcela, Tavares R. Marina, Červený Jakub, Vlachová Miluše, Šírová Milada, Pelantová Helena, Petrásková Lucie, Bumba Ladislav, Konefal Rafal, Etrych Tomáš, Křen Vladimír, Chytil Petr, Bojarová Pavla: Glycopolymers Decorated with 3‑O-Substituted Thiodigalactosides as Potent Multivalent Inhibitors of Galectin‑3. Journal of Medicinal Chemistry, 65 (5), 3866-3878 (2022). 
  • Pavlína Nekvasilová, Michaela Hovorková, Zuzana Mészáros, Lucie Petrásková, Helena Pelantová, Vladimír Křen, Kristýna Slámová, Pavla Bojarová: Engineered Glycosidases for the Synthesis of Analogs of Human Milk Oligosaccharides. International Journal of Molecular Sciences, 23 (8), 4106:1-13 (2022). 
  • Lucie Petrásková, Kristýna Káňová, Katerina Brodsky, Anastasiia Hetman, Barbora Petránková, Helena Pelantová, Vladimír Křen, Kateřina Valentová: Sulfated Phenolic Substances: Preparation and Optimized HPLC Analysis. International Journal of Molecular Sciences, 23 (10), 5743:1-18 (2022). 
  • Zuzana Mészáros, Lucie Petrásková, Natalia Kulik, Helena Pelantová, Pavla Bojarová, Vladimír Křen, Kristýna Slámová: Hypertransglycosylating Variants of the GH20 β-N-Acetylhexosaminidase for the Synthesis of Chitooligomers. Advanced Synthesis & Catalysis, 364 (12), 2009-2022 (2022). 
  • Katerina Brodsky, Kristýna Káňová, Dorota Konvalinková, Kristýna Slámová, Helena Pelantová, Kateřina Valentová, Pavla Bojarová, Vladimír Křen, Lucie Petrásková: Bacterial Aryl Sulfotransferases in Selective and Sustainable Sulfation of Biologically Active Compounds using Novel Sulfate Donors. ChemSusChem, 15 (18), e202201253: 1-9 (2022). 
  • Pavlína Nekvasilová, Kulik Natalia, Michael Kotík, Lucie Petrásková, Kristýna Slámová, Vladimír Křen, Pavla Bojarová: Mutation Hotspot for Changing the Substrate Specificity of β-N-Acetylhexosaminidase: A Library of GlcNAcases. International Journal of Molecular Sciences, 23 (20), 12456: 1-17 (2022). 
  • Viola Kolaříková, Katerina Brodsky, Lucie Petrásková, Helena Pelantová, Josef Cvačka, Libor Havlíček, Vladimír Křen, Kateřina Valentová: Sulfation of Phenolic Acids: Chemoenzymatic vs. Chemical Synthesis. International Journal of Molecular Sciences, 23 (23), 15171: 1-16 (2022). 
  • Low Dorrain Yanwen, Micheau Pierre, Koistinen Mikael Ville, Hanhineva Kati, Valentová Kateřina, Petrásková Lucie, et a..: Data sharing in PredRet for accurate prediction of retention time: Application to plant food bioactive compounds. Food Chemistry, 357, 129757 1-10 (2021). 
  • Heine Viktoria, Hovorková Michaela, Vlachová Miluše, Filipová Marcela, Bumba Ladislav, Janoušková Olga, Hubálek Martin, Cvačka Josef, Petrásková Lucie, Pelantová Helena, Křen Vladimír, Elling Lothar, Bojarová Pavla: Immunoprotective neo-glycoproteins: Chemoenzymatic synthesis of multivalent glycomimetics for inhibition of cancer-related galectin-3. European Journal of Medicinal Chemistry, 220, 113500, 1-10 (2021). 
  • Michaela Hovorková, Natalia Kulik, Dorota Konvalinková, Lucie Petrásková, Vladimír Křen, Pavla Bojarová: Mutagenesis of Catalytic Nucleophile of β-Galactosidase Retains Residual Hydrolytic Activity and Affords a Transgalactosidase. ChemCatChem, 13 (21), 4532-4542 (2021).
  • Petrásková Lucie, Káňová Kristýna, Biedermann David, Křen Vladimír, Valentová Kateřina: Simple and Rapid HPLC Separation and Quantification of Flavonoid, Flavonolignans, and 2,3-Dehydroflavonolignans in Silymarin. Foods, 9 (2), 116:1-14 (2020).
  • Valentová Kateřina, Havlík Jaroslav, Kosina Pavel, Papoušková Barbora, Jaimes José Diógenes, Káňová Kristýna, Petrásková Lucie, Ulrichová Jitka, Křen Vladimír: Biotransformation of Silymarin Flavonolignans by Human Fecal Microbiota. Metabolites, 10 (1), 29:1-16 (2020).
  • Nekvasilová Pavlína, Andreasová Iveta, Petrásková Lucie, Pelantová Helena, Křen Vladimír, Bojarová Pavla: A novel enzymatic tool for transferring GalNAc moiety onto challenging acceptors. BBA – Proteins and Proteomics, 1868 (2), 140319:1-8 (2020). 
  • Simona Dobiasová, Kateřina Řehořová, Denisa Kučerová, David Biedermann, Kristýna Káňová, Lucie Petrásková, Kamila Koucká, Radka Václavíková, Kateřina Valentová, Tomáš Ruml, Tomáš Macek, Vladimír Křen, Jitka Viktorová: Multidrug Resistance Modulation Activity of Silybin Derivatives and Their Anti-inflammatory Potential. Antioxidants, 9 (5), 455:1-25 (2020). 
  • Jana Kapešová, Lucie Petrásková, Natalia Kulik, Zuzana Straková, Pavla Bojarová, Kristína Markošová, Martin Rebroš, Vladimír Křen, Kristýna Slámová: Transglycosidase activity of glycosynthase-type mutants of a fungal GH20 β-N-acetylhexosaminidase. International Journal of Biological Macromolecules, 161, 1206-1215 (2020). 
  • Tomáš Vašíček, Vojtěch Spiwok, Jakub Červený, Lucie Petrásková, Ladislav Bumba, David Vrbata, Helena Pelantová, Vladimír Křen, Pavla Bojarová: Regioselective 3-O-Substitution of Unprotected Thiodigalactosides: Direct Route to Galectin Inhibitors. Chemistry-A European Journal, 26 (43), 9620-9631 (2020). 
  • Lenka Rucká, Natalia Kulik, Petr Novotný, Anastasia Sedova, Lucie Petrásková , Romana Příhodová, Barbora Křístková, Petr Halada, Miroslav Pátek, Ludmila Martínková: Plant Nitrilase Homologues in Fungi: Phylogenetic and Functional Analysis with Focus on Nitrilases in Trametes versicolor and Agaricus bisporus. Molecules, 25 (17), 3861: 1-20 (2020). 
  • Lydie Plecitá Hlavatá, Hana Engstová, Blanka Holendová, Jan Tauber, Tomáš Špaček, Lucie Petrásková, Vladimír Křen, Jitka Špačková, Klára Gotvaldová, Jan Ježek, Andrea Dlasková, Katarína Smolková, Petr Ježek: Mitochondrial Superoxide Production Decreases on Glucose-Stimulated Insulin Secretion in Pancreatic β Cells Due to Decreasing Mitochondrial Matrix NADH/NAD+ Ratio. Antioxidants & Redox Signaling, 33 (12), 789-815 (2020).   
  • Ludmila Martínková, Romana Příhodová, Natalia Kulik, Helena Pelantová, Barbora Křístková, Lucie Petrásková, David Biedermann: Biocatalyzed Reactions towards Functional Food Components 4-Alkylcatechols and Their Analogues. Catalysts, 10 (9), 1077: 1-14 (2020). 
  • Pavlína Nekvasilová, Natalia Kulik, Nikola Rychlá, Helena Pelantová, Lucie Petrásková, Zuzana Bosáková, Josef Cvačka, Kristýna Slámová, Vladimír Křen, Pavla Bojarová: How Site-Directed Mutagenesis Boosted Selectivity of a Promiscuous Enzyme. Advanced Synthesis and Catalysis, 362 (19), 4138-4150 (2020). 
  • Kristýna Káňová, Lucie Petrásková, Helena Pelantová, Zuzana Rybková, Kateřina Malachová, Josef Cvačka, Vladimír Křen, Kateřina Valentová: Sulfated Metabolites of Luteolin, Myricetin, and Ampelopsin: Chemoenzymatic Preparation and Biophysical Properties. Journal of Agricultural and Food Chemistry, 68 (40), 11197-11206 (2020).
  • Biedermann David, Moravcová Veronika, Valentová Kateřina, Kuzma Marek, Petrásková Lucie, Císařová Ivana, Křen Vladimír: Oxidation of flavonolignan silydianin to unexpected lactone-acid derivative. Phytochemistry Letters, 30, 14-20 (2019). 
  • Šuk Jakub, Jašprová Jana, Biedermann David, Petrásková Lucie, Valentová Kateřina, Křen Vladimír, Muchová Lucie, Vítek Libor: Isolated Silymarin Flavonoids Increase Systemic and Hepatic Bilirubin Concentrations and Lower Lipoperoxidation in Mice. Oxidative Medicine and Cellular Longevity, Volume 2019, Article ID 6026902, 1-12 (2019).
  • Kapešová Jana, Petrásková Lucie, Markošová Kristína, Rebroš Martin, Kotík Michael, Bojarová Pavla, Křen Vladimír: Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of „Solid State Biocatalysis“. International Journal of Molecular Sciences, 20 (5), 1112 (1-15) (2019). 
  • Bassanini Ivan, Kapešová Jana, Petrásková Lucie, Pelantová Helena, Markošová Kristína, Rebroš Martin, Valentová Kateřina, Kotík Michael, Káňová Kristýna, Bojarová Pavla, Cvačka Josef, Turková Lucie, Ferrandi E. Erica, Bayout Ikram, Riva Sergio, Křen Vladimír: Glycosidase-Catalyzed Synthesis of Glycosyl Esters and Phenolic Glycosides of Aromatic Acids. Advanced Synthesis and Catalysis, 361 (11), 2627-2637 (2019). 
  • Plecitá-Hlavatá Lydie, Engstová Hana, Ježek Jan, Holendová Blanka, Tauber Jan, Petrásková Lucie, Křen Vladimír, Ježek Petr: Potential of Mitochondria-Targeted Antioxidants to Prevent Oxidative Stress in Pancreatic β-cells. Oxidative Medicine and Cellular Longevity, Volume 2019, ID 1826303, 1-16 (2019). 
  • Begines Paloma, Biedermann David, Valentová Kateřina, Petrásková Lucie, Pelantová Helena, Maya Inés, Fernández-Bolaños G. José, Křen Vladimír: Chemoenzymatic Synthesis and Radical Scavenging of Sulfated Hydroxytyrosol, Tyrosol, and Acetylated Derivatives. Journal of Agricultural and Food Chemistry, 67 (26), 7281-7288 (2019). 
  • Chambers S. Christopher, Biedermann David, Valentová Kateřina, Petrásková Lucie, Viktorová Jitka, Kuzma Marek, Křen Vladimír: Preparation of Retinoyl-Flavonolignan Hybrids and Their Antioxidant Properties. Antioxidants, 8 (7), 236:1-12 (2019). 
  • Pourová Jana, Applová Lenka, Macáková Kateřina, Vopršalová Marie, Migkos Thomas, Bentanachs Roger, Biedermann David, Petrásková Lucie, Tvrdý Václav, Hrubša Marcel, Karlíčková Jana, Křen Vladimír, Valentová Kateřina, Mladěnka Přemysl: The Effect of Silymarin Flavonolignans and Their Sulfated Conjugates on Platelet Aggregation and Blood Vessels Ex Vivo. Nutrients, 11 (10), 2286: 1-16 (2019). 
  • Rucká Lenka, Chmátal Martin, Kulik Natalia, Petrásková Lucie, Pelantová Helena, Novotný Petr, Příhodová Romana, Pátek Miroslav, Martínková Ludmila: Genetic and Functional Diversity of Nitrilases in Agaricomycotina. International Journal of Molecular Sciences, 20 (23), 5990: 1-19 (2019). 
  • Garcia-Oliva Cecilia, Hoyos Pilar, Petrásková Lucie, Kulik Natalia, Pelantová Helena, Cabanillas H. Alfredo, Rumbero Ángel, Křen Vladimír, Hernáiz J. María, Bojarová Pavla: Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation. International Journal of Molecular Sciences, 20 (24), 6181: 1-17 (2019). 
  • Rädisch Robert, Chmátal Martin, Rucká Lenka, Novotný Petr, Petrásková Lucie, Halada Petr, Kotík Michael, Pátek Miroslav, Martínková Ludmila: Overproduction and characterization of the first enzyme of a new aldoxime dehydratase family in Bradyrhizobium sp. International Journal of Biological Macromolecules, 115, 746-753 (2018). 
  • Valentová Kateřina, Purchartová Kateřina, Rydlová Lenka, Roubalová Lenka, Biedermann David, Petrásková Lucie, Křenková Alena, Pelantová Helena, Holečková-Moravcová Veronika, Tesařová Eva, Cvačka Josef, Vrba Jiří, Ulrichová Jitka, Křen Vladimír: Sulfated Metabolites of Flavonolignans and 2,3-Dehydroflavonolignans: Preparation and Properties. International Journal of Molecular Sciences, 19 (8), 2349 (1-17) (2018). 
  • Koistinen Ville Mikael, Da Silva Andreia Bento, Abrankó László, Low Dorrain, Villalba Rocio Garcia, Barberán Francisco Tomás, Landberg Rikard, Savolainen Otto, Alvarez-Acero Inmaculada, De Pascual-Teresa Sonia, Van Poucke Christof, Almeida Conceicao, Petrásková Lucie, Valentová Kateřina et al.: Interlaboratory Coverage Test on Plant Food Bioactive Compounds and their Metabolites by Mass Spectrometry-Based Untargeted Metabolomics. Metabolites, 8 (3), 46 (1-17) (2018). 
  • Bojarová P., Tavares M.R., Laaf D., Bumba L., Petrásková L., Konefal R., Bláhová M., Pelantová H., Elling L., Etrych T., Chytil P., Křen V.: Biocompatible glyconanomaterials based on HPMA-copolymer for specific targeting of galectin-3. Journal of Nanobiotechnology, 16:73, (1-16) (2018). 
  • Bojarová P., Chytil P., Mikulová B., Bumba L., Konefal R., Pelantová H., Krejzová J., Slámová K., Petrásková L., Kotrchová L., Cvačka J., Etrych T., Křen V.: Glycan-decorated HPMA copolymers as high-affinity lectin ligands. Polymer Chemistry, 8 (17), 2647-2658 (2017). 
  • Heřmánková-Vavříková E., Křenková A., Petrásková L., Chambers C. S., Zápal J., Kuzma M., Valentová K., Křen V.: Synthesis and Antiradical Activity of Isoquercitrin Esters with Aromatic Acids and Their Homologues. International Journal of Molecular Sciences, 18 (5), 1074 (1-14) (2017).
  • Chambers S.C., Holečková V., Petrásková L., Biedermann D., Valentová K., Buchta M., Křen V.: The silymarin composition… and why does it matter??? Food Research International, 100 (3), 339-353 (2017). 
  • Valentová K., Káňová K., Di Meo F., Pelantová H., Chambers S.C., Rydlová L., Petrásková L., Křenková A., Cvačka J., Trouillas P., Křen V.: Chemoenzymatic Preparation and Biophysical Properties of Sulfated Quercetin Metabolites. International Journal of Molecular Sciences, 18 (11), 2231 (2017). 

Coordinated Projects

  • Czech Science Foundation – GA23-05146S – Study of galectin-glycan interactions using fluoroglycomimetics (2023-2025)
  • Czech Science Foundation – GA19-00043S „Aryl-sulfotransferases and their application in the preparation of sulfated metabolites“ (2019-2021)

Hobbies

  • outdoor activities (biking, hiking)
  • gardening

 

Last update: October 3, 2023