Ing. Ludmila Martínková, CSc. DSc.

Senior Scientist
(+420) 296 442 569
Laboratory of Biotransformation
Institute of Microbiology of the Czech Academy of Sciences
Vídeňská 1083
Praha 4
142 20
Czech Republic


  • 92 articles in peer-reviewed journals (Web of Science)
  • 9 book chapters
  • citation index (with autocitations): 2905 (Web of Science)
  • h-index: 33 (Web of Science)

ORCID: 0000-0002-1937-7081

Research interests

  • Nitrilases, cyanide hydratases
  • Aldoxime dehydratases
  • Tyrosinases, laccases
  • Genome mining, overproduction of enzymes
  • Biotransformation (aldoximes, nitriles, phenols)
  • Biodegradation (cyanides,nitriles, phenols)
  • Valorization of lignin monomers
  • Product analysis (HPLC)


  • Institute of Chemical Technology, Prague (Fermentation Technology and Bioengineering) – MSc (1984)

Scientific degrees

  • Czechoslovak Academy of Sciences, Institute of Microbiology – PhD in biology/micro­biology (1989)
  • Czech Academy of Sciences – DSc. (= Res. Prof.) award (2018) – Thesis “Microbial Enzyme Transformations of Cyano and Phenolic Compounds: Advances, Challenges and Perspectives”

International experience

  • 1990 (1 month) Institute of Biochemistry, TU Wien (FEMS Fellowship)
  • 1995, 1997 (total 3 months) Institute of Microbiology, University of Stuttgart (supported by DAAD Fellowship and Univ. Stuttgart)
  • 1999-2000 short-term stays at Institute of Organic Chemistry, TU Graz, Institute of Organic Chemistry, University of Debrecen, Institute of Organic Chemistry, and University of Hamburg
  • selected international collaborations: Dr. Margit Winkler, Dr. Norbert Klempier, TU Graz, Austria; Prof. Florian Rudroff, TU Wien, Austria; Prof. Jean Bolte, University of Clermont-Ferrand, France; Prof. Maria Cantarella, University of L´Aquila, Italy; Prof. Herfried Griengl, TU Graz, Austria; Prof. Lutz Fischer, University of Hohenheim – Stuttgart, Germany; Dr. Giovanni Nicolosi, CNR, Catania, Italy; Prof. Marius Réglier, University of Aix Marseille, France; Dr. Andreas Stolz, University of Stuttgart, Germany
  • COST Management Committees Member in Actions D25, CM0701, CM1003 and CA17128; WG coordinator in Action D25

Selected recent projects

  • 2022-2024 Czech Science Foundation (CSF), GA22-06785S Free-cyanide determination: new approaches based on enzyme cascades. Project coordinator.
  • 2020–2022 CSF – FWF (Austria) – Lead Agency Project GF20-23532L  A new chemoenzymatic route from carboxylic acids to nitriles. Team member, working group coordinator.
  • 2019–2022 MEYS, CZ – LTC19037 Valorization of lignin using fungal oxidases. Project coordinator.
  • 2018–2020 MEYS, CZ – LTC18080 Functionalization of C-H bonds in phenol compounds by tyrosinases. Project coordinator.
  • 2018–2020 Czech Science Foundation (CSF), GA18-00184S New nitrilase superfamily proteins in Basidiomycota. Project coordinator.
  • 2014–2017 Agency of Technology, CZ – TA04021212 Development of technology for removal of organic substances from coking plant sewage. Coordinator.

Selected recent publications

  • Ludmila Martínková*, Natalia Kulik, Lenka Rucká, Michael Kotik, Barbora Křístková, Katarína Šťastná, Petr Novotný, Romana Příhodová, Pavla Bojarová, Miroslav Pátek: Biotransformation of free cyanide to formic acid by a cyanide hydratase-formamidase cascade reaction. Process Biochemistry, 142, 6 April 2024, 62-67 (2024).
  • Barbora Křístková, Ludmila Martínková*, Lenka Rucká, Michael Kotík, Natalia Kulik, Robert Rädish, Margit Winkler, Miroslav Pátek: Immobilization of aldoxime dehydratases on metal affinity resins and use of the immobilized catalysts for the synthesis of nitriles important in fragrance industry. Journal of Biotechnology, 384, 20 March 2024, 12-19 (2024).
  • Dominic Goj, Stella Ebner, Melissa Horvat, Simon Arhar, Ludmila Martínková, Margit Winkler: Cell-free reduction of carboxylic acids with secreted carboxylic acid reductase. Journal of Biotechnology, 382 (February 2024), 44-50 (2024). IF 4,1.
  • Ludmila Martínková*, Michal Grulich, Miroslav Pátek, Barbora Křístková, Margit Winkler: Bio-Based Valorization of Lignin-Derived Phenolic Compounds: A Review. Biomolecules, 13 (5), 717: 1-25 (2023). IF 6,064.
  • Ludmila Martínková*, Natalia Kulik, Anastasia Sedova, Barbora Křístková, Pavla Bojarová: Recent Progress in the Production of Cyanide-Converting Nitrilases-Comparison with Nitrile-Hydrolyzing Enzymes. Catalysts, 13 (3), 500:1-19 (2023). IF 4,501.
  • Margit Winkler, Horvat Melissa, Schiefer Astrid, Weilch Victoria, Rudroff Florian, Pátek Miroslav, Martínková Ludmila*: Organic Acid to Nitrile: A Chemoenzymatic Three-Step Route. Advanced Synthesis & Catalysis, 365 (1), 37-42 (2023). IF 5,981.
  • Ludmila Martínková*, Pavla Bojarová, Anastasia Sedova, Vladimír Křen: Recent trends in the treatment of cyanide-containing effluents: Comparison of different approaches. Critical reviews in environmental science and technology, 53 (3), 416-434 (2023). IF 11,750.
  • Barbora Křístková, Robert Rädisch, Natalia Kulik, Melissa Horvat, Lenka Rucká, Milan Grulich, Florian Rudroff, Alan Kádek, Miroslav Pátek, Margit Winkler, Ludmila Martínková*: Scanning aldoxime dehydratase sequence space and characterization of a new aldoxime dehydratase from Fusarium Vanettenii. Enzyme and Microbial Technology, 164, 110187:1-13 (2023). IF 3,705.
  • Ludmila Martínková*, Barbora Křístková, Vladimír Křen: Laccases and Tyrosinases in Organic Synthesis. International Journal of Molecular Sciences, 23 (7), 3462:1-19 (2022).  IF 6,208.
  • Robert Rädisch, Miroslav Pátek, Barbora Křístková, Margit Winkler, Vladimír Křen, Ludmila Martínková*: Metabolism of Aldoximes and Nitriles in Plant-Associated Bacteria and Its Potential in Plant-Bacteria Interactions. Microorganisms, 10 (3), 549:1-16 (2022).  IF 4,926.
  • Melissa Horvat, Victoria Weilch, Robert Rädisch, Sebastian Hecko, Astrid Schiefer, Florian Rudroff, Birgit Wilding, Norbert Klempier, Miroslav Pátek, Ludmila Martínková*, Margit Winkler: Chemoenzymatic one-pot reaction from carboxylic acid to nitrile via oxime†.
    Catalysis Science & Technology, 12 (1), 62-66 (2022). IF 6,177.
  • Sedova A., Rucká L., Bojarová P., Glozlová M., Novotný P., Křístková B., Pátek M., Martínková L.*: Application Potential of Cyanide Hydratase from Exidia Glandulosa: Free Cyanide Removal from Simulated Industrial Effluents. Catalysts, 11 (11), 1410:1-16 (2021). IF 4,146.
  • Rucká L., Kulik N., Novotný P., Sedova A., Petrásková L., Příhodová R., Křístková B., Halada P., Pátek M., Martínková L.*: Plant nitrilase homologues in fungi: Phylogenetic and functional analysis with focus on vitrilases in Trametes versicolor and Agaricus bisporus. Molecules 25, art. no. 3861 (2020). IF 3,060.
  • Martínková L., Příhodová R., Kulik N., Pelantová H., Křístková B., Petrásková L., Biedermann D.: Biocatalyzed reactions towards functional food components 4-alkylcatechols and their analogues. Catalysts 10, art. no. 1077 (2020). IF 4,146.
  • Martínková L. *: Nitrile metabolism in fungi: A review of its key enzymes nitrilases with focus on their biotechnological impact.* Fung. Biol. Rev. 33, 149-157 (2019). IF 5.563
  • Rucká L., Chmátal M., Kulik N., Petrásková L., Pelantová H., Novotný P., Příhodová R., Pátek M., Martínková L.*: Genetic and functional diversity of nitrilases in Agaricomycotina. Inst. J. Mol. Sci. 20, art. no. 5990 (2019). IF 4.784
  • Rädisch R., Chmátal M., Rucká L., Novotný P., Petrásková L, .Halada P., Kotik, M., Pátek, M., Martínková L.*: Overproduction and characterization of the first enzyme of a new aldoxime dehydratase family in Bradyrhizobium sp. Int. J. Sci. Macromol. 115, 746-753 (2018). IF 4,784
  • Martínková L., Křen V.: Biocatalytic production of mandelic acid and analogues: a review and comparison with chemical processes. Applied Microbiology and Biotechnology, 102 (9), 3893-3900 (2018). IF 3,340.
  • Martínková L.*, Kotik M., Marková E., Homolka L.: Biodegradation of phenolic compounds by Basidiomycota and its phenol oxidases: A review. Chemosphere 149, 373–382 (2016). IF (2016) 3.340
  • Marková E., Kotik M., Křenková A., Man P., Haudecoeur R., Boumendjel A., Hardré R., Mekmouche Y., Courvoisier-Dezord E., Réglier M., Martínková L.*: Recombinant tyrosinase from Polyporus arcularius: Overproduction in Escherichia coli, characterization, and use in a study of aurones as tyrosinase effectors. J. Agric. Food Chem. 64, 2925−2931 (2016). IF (2016) 2.912
  • Martínková L.*, Chmátal M.: The integration of cyanide hydratase and tyrosinase catalysts enables effective degradation of cyanide and phenol in coking wastewaters. Wat. Res. 102, 90–95 (2016). IF (2016) 7.913

*corresponding author

Pedagogical activities

  • Supervision of students who obtained PhD (9)
  • Supervision of undergraduate students (>30)
  • Reviews of PhD theses (Charles Univ. in Prague, Univ. of Sydney, Univ. of Cape Town)
  • member of the Postgraduate Program Biochemistry and Bioorganic Chemistry (ICT)

Research organization activities

  • Evalution Committee member in Horizon Europe (2020 – 2024)
  • COST MC Member in Actions D25, CM0701, CM1003 and CA17128
  • Remote Evaluator in Horizon 2020 (2014 – 2018)
  • Evaluator in Horizon 2020 (2016 – 2020)
  • Evaluator in Horizon Europe (2021)
  • COST MC Member in Actions D25, CM0701 and CM1003
  • Member of symposium organization committees (Biotrans 2003 Olomouc, Biocat 2006 Hamburg)
  • Guest Editor – Special Issue „Nitrilases and Nitrile Hydratases“ (Molecules, 2020) – with Dr. Margit Winkler (TU Graz)
  • Member of Editorial Board – Biocatalysis and Biotransformation
  • Reviewer for journals Advanced Synthesis&Catalysis, Applied and Environmental Microbiology, Applied Microbiology and Biotechnology, Biotechnology Advances,  BMC Biotechnology, Ecology and Environmental Safety, Extremophiles, Journal of Agricultural and Food Chemistry, Molecules etc.


  • Czechoslovak Society for Microbiology
  • Czech Society for Biochemistry and Molecular Biology
  • Czech Chemical Society


  • Czech (mother tongue)
  • English, German (fluent)
  • French, Italian, Russian, Latin (basic)


Last update June 13, 2024