Ing. Ludmila Martínková, CSc. DSc.

Position:
Senior Scientist
Email:
martinko@biomed.cas.cz
Phone:
(+420) 296 442 569
Address:
Laboratory of Biotransformation
Institute of Microbiology of the Czech Academy of Sciences
Vídeňská 1083
Praha 4
142 20
Czech Republic

Education

  • Institute of Chemical Technology, Prague (Fermentation Technology and Bioengineering) – MSc (1984)
  • Czechoslovak Academy of Sciences, Institute of Microbiology – PhD in biology/micro­biology (1989)
  • Czech Academy of Sciences – DSc. (= Res. Prof.) award (2018) – Thesis “Microbial Enzyme Transformations of Cyano and Phenolic Compounds: Advances, Challenges and Perspectives”

Research Orientation

  • Nitrilases, cyanide hydratases
  • Tyrosinases, laccases
  • Genome mining, overproduction of enzymes
  • Biotransformation (aldoximes, nitriles, phenols)
  • Biodegradation (cyanides,nitriles, phenols)
  • Valorization of lignin monomers
  • Product analysis (HPLC)

International Experience

  • 1990 (1 month) Institute of Biochemistry, TU Wien (FEMS Fellowship)
  • 1995, 1997 (total 3 months) Institute of Microbiology, University of Stuttgart (supported by DAAD Fellowship and Univ. Stuttgart)
  • 1999-2000 short-term stays at Institute of Organic Chemistry, TU Graz, Institute of Organic Chemistry, University of Debrecen, Institute of Organic Chemistry, and University of Hamburg
  • selected international collaborations: Prof. Jean Bolte, University of Clermont-Ferrand, France; Prof. Maria Cantarella, University of L´Aquila, Italy; Prof. Herfried Griengl, TU Graz, Austria; Prof. Lutz Fischer, University of Hohenheim – Stuttgart, Germany; Dr. Margit Winkler and Dr. Norbert Klempier, TU Graz, Austria; Dr. Giovanni Nicolosi, CNR, Catania, Italy; Prof. Marius Réglier, University of Aix Marseille, France; Dr. Andreas Stolz, University of Stuttgart, Germany
  • COST Management Committees Member in Actions D25, CM0701, CM1003 and CA17128; WG coordinator in Action D25

Membership

  • Czechoslovak Society for Microbiology
  • Czech Society for Biochemistry and Molecular Biology
  • Czech Chemical Society

Languages

  • Czech (mother tongue)
  • English, German (fluent)
  • French, Italian, Russian, Latin (basic)

Coordinated Projects

  • 2020–2022 CSF – FWF (Austria) – Lead Agency Project GF20-23532L  A new chemoenzymatic route from carboxylic acids to nitriles. Working group coordinator.
  • 2019–2022 MEYS, CZ – LTC19037 Valorization of lignin using fungal oxidases. Project coordinator.
  • 2018–2020 MEYS, CZ – LTC18080 Functionalization of C-H bonds in phenol compounds by tyrosinases. Project coordinator.
  • 2018–2020 Czech Science Foundation (CSF), GA18-00184S New nitrilase superfamily proteins in Basidiomycota. Project coordinator.
  • 2014–2017 Agency of Technology, CZ – TA04021212 Development of technology for removal of organic substances from coking plant sewage. Working group coordinator (co-applicant)
  • 2011–2015 Ministry of Education, Youth and Sports (MEYS), CZ – LD12049 Characterization and application of fungal non-heme oxidases. Project coordinator.
  • Technology Agency of the Czech Republic – TA010211368 „Biological elimination of cyanide from industrial wastewaters with complex pollution“ (2011–2014)
  • Czech Science Foundation – GAP504/11/0394 „Interspecies comparison of nitrilases by using recombinant enzymes obtained by database-mining: their environmental impact and potential application” (2011–2014)
  • IAA500200708 „Nitrilases from filamentous fungi: cloning, expression, characterization and directed evolution“ (2007–2011)
  • COST OC09046 „Cascade chemoenzymatic reactions of nitriles“ (2006–2011)
  • FT-TA5/043 „Research of innovative techniques for elimination of benzonitrile pesticides from the environment“ (2006–2010)
  • NPV II 2B06151 „Biodegradation of polybrominated compounds…“ (2006–2010)
  • IAA500200708 „Nitrilases from filamentous fungi: cloning, expression, characterization and directed evolution“ (Grant Agency of the Czech Academy of Sciences) (2007–2011)
  • 2B06151 „Biodegradation of polybrominated compounds“ (Ministry of Education, Youth and Sports of the Czech Republic) (2006–2010)
  • Action CASCAT CM07010, Cascade Chemoenzymatic Processes – New Synergies Between Chemistry and
  • Biochemistry, Working group 3, Cascade reactions at the nitrile group (COST, European Science Foundation;(2008–201­1)

Publications

  • 86 articles and book chapters

      Citation index (with autocitations): 2564

      h-index: 28 (according to Web of Science – All databases)

Selected recent publications

  • Rinágelová A., Kaplan O., Veselá A.B., Chmátal M., Křenková A., Plíhal O., Pasquarelli F., Cantarella M., Martínková L.*: Cyanide hydratase from Aspergillus niger K10: Overproduction in Escherichia coli, purification, characterization and use in continuous cyanide degradation. Proc. Biochem. 49, 445–450 (2014). IF (2014) 2.516
  • Martínková L.*, Veselá A.B., Rinágelová A., Chmátal M.:. Cyanide hydratases and cyanide dihydratases: emerging tools in the biodegradation and biodetection of cyanide. Appl. Microbiol. Biotechnol. 99, 8875–8882 (2015). IF (2015) 3.337
  • Veselá, A.B., Rucká, L., Kaplan, O., Pelantová, H., Nešvera, J., Pátek, M., Martínková, L.*: Bringing nitrilase sequences from databases to life: the search for novel substrate specificities with a focus on dinitriles. Appl. Microbiol. Biotechnol. 100, 2193–2202 (2016). IF (2015) 3.67
  • Martínková L.*, Kotik M., Marková E., Homolka L.: Biodegradation of phenolic compounds by Basidiomycota and its phenol oxidases: A review. Chemosphere 149, 373–382 (2016). IF (2016) 3.340
  • Marková E., Kotik M., Křenková A., Man P., Haudecoeur R., Boumendjel A., Hardré R., Mekmouche Y., Courvoisier-Dezord E., Réglier M., Martínková L.*: Recombinant tyrosinase from Polyporus arcularius: Overproduction in Escherichia coli, characterization, and use in a study of aurones as tyrosinase effectors. J. Agric. Food Chem. 64, 2925−2931 (2016). IF (2016) 2.912
  • Martínková L.*, Chmátal M.: The integration of cyanide hydratase and tyrosinase catalysts enables effective degradation of cyanide and phenol in coking wastewaters. Wat. Res. 102, 90–95 (2016). IF (2016) 7.913
  • Rädisch R., Chmátal M., Rucká L., Novotný P., Petrásková L, .Halada P., Kotik, M., Pátek, M., Martínková L.*: Overproduction and characterization of the first enzyme of a new aldoxime dehydratase family in Bradyrhizobium sp. Int. J. Sci. Macromol. 115, 746-753 (2018). IF 4,784
  • Martínková L. *: Nitrile metabolism in fungi: A review of its key enzymes nitrilases with focus on their biotechnological impact.* Fung. Biol. Rev. 33, 149-157 (2019). IF 5.563
  • Rucká L., Chmátal M., Kulik N., Petrásková L., Pelantová H., Novotný P., Příhodová R., Pátek M., Martínková L*: Genetic and functional diversity of nitrilases in Agaricomycotina. Inst. J. Mol. Sci. 20, art. no. 5990 (2019). IF 4.784

*corresponding author

Pedagogical activities

  • Supervision of students who obtained PhD (8)
  • Reviews of PhD theses (Charles Univ. in Prague, Univ. of Sydney, Univ. of Cape Town)
  • member of the Postgraduate Program Biochemistry and Bioorganic Chemistry (ICT)

Research organization activities

  • Member of evaluation panels in H2020 (2016 – 2020)
  • COST MC Member in Actions D25, CM0701 and CM1003
  • Member of symposium organization committees (Biotrans 2003 Olomouc, Biocat 2006 Hamburg)
  • Member of Editorial Boards (Biotechnology Letters, Biocatalysis and Biotransformation)
  • Reviewer for journals Advanced Synthesis&Catalysis, Applied and Environmental Microbiology, Applied Microbiology and Biotechnology, Biotechnology Advances,  BMC Biotechnology, Ecology and Environmental Safety, Extremophiles, Journal of Agricultural and Food Chemistry, Molecules etc.

 

Last update 09/04/2020