RNDr. Pavla Bojarová, PhD.

Position:
Senior Scientist
Email:
bojarova@biomed.cas.cz
pavla.bojarova@fbmi.cvut.cz
Phone:
(+420) 296 442 360
Address:
Laboratory of Biotransformation
Institute of Microbiology of the Czech Academy of Sciences
Vídeňská 1083
Praha 4 – Krč
142 20
Czech Republic

Education

  • Charles University Prague, Faculty of Sciences, Dept. of Biochemistry – MSc (2002)
  • Charles University Prague, Faculty of Sciences, Dept. of Biochemistry – PhD (2006)

PhD Thesis

  • Modified Substrates in β-N-Acetylhexosami­nidase-Catalyzed Synthesis
  • PhD. thesis summary (240kB)

Employment

Since 2019 Lecturer at Faculty of Biomedical Engineering, Czech Technical University in Prague
Since 2010 Lecturer at Department of Biochemistry, Faculty of Science, Charles University in Prague (Biochemistry and Biomedicine of Carbohydrates)
Since 2008 Research fellow in the Laboratory of Biotransformation
2006–2007 Postdoctoral stay at the University of Melbourne, Australia
Since 1999 member of the Laboratory of Biotransformation

Study stays

Year Institute Length
2010 Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen 2 weeks
2006–7 School of Chemistry, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Australia 13 mths
2006 Directed Enzyme Evolution Course, Inst. Biotechnology, Vilnius 1 week
2005 Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen 7 weeks
2005 Istituto di Chimica del Riconoscimento Molecolare, CNR, Milan 4 weeks
2003 Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen 3 mths
2003 Departamento de Quimica organica, Fac.de Quimica, Univ. de Sevilla 4 weeks
2002 Institut für Organische Chemie, Universität Hamburg 4 weeks

Publications & Presentations

  • 40 papers
  • 4 book chapters
  • 1 patent application
  • 935 citations (Web of Science, All databases)
  • H-index 18
  • (Co-)author of over 40 oral or poster presentations in international conferences

Selected publications (last 10 years)

  • P. Bojarová a S. J. Williams. Aryl sulfamates are broad spectrum inactivators of sulfatases: Effects on sulfatases from various sources. Bioorg. Med. Chem. Lett. 2009, 19, 477–480.
  • K. J. Loft, P. Bojarová, K. Slámová, V. K en a S. J. Williams. Synthesis of sulfated glucosaminides for profiling substrate specificities of sulfatases and fungal b-N-acetylhexosami­nidases. ChemBioChem 2009, 10, 565–576.
  • P. Bojarová a V. Křen. Glycosidases: a key to tailored carbohydrates. Trends Biotechnol. 2009, 27, 199–209.
  • K. Slámová, R. Gažák, P. Bojarová, N. Kulik, R. Ettrich, H. Pelantová, P. Sedmera a V. Křen. 4-Deoxy-substrates for β-N-acetylhexosami­nidases: how to make use of their loose specificity. Glycobiology 2010, 20, 1002–1009.
  • K. Slámová, P. Bojarová, L. Petrásková a V. Křen. b-N-Acetylhexosami­nidase: what’s in a name…? Biotechnol. Adv. 2010, 28, 682–693.
  • P. Bojarová a V. Křen. Glycosidases in carbohydrate synthesis: when organic chemistry falls short. Chimia 2011, 65, 65–70.
  • H. Ryšlavá, A. Kalendová, V. Doubnerová, P. Skočdopol, V. Kumar, Z. Kukačka, P. Pompach, O. Vaněk, K. Slámová, P. Bojarová, N. Kulik, R. Ettrich, V. Křen a K. Bezouška. Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosami­nidase. FEBS J. 2011, 278, 2469–2484.
  • P. Bojarová, K. Slámová, K. Křenek, R. Gažák, N. Kulik, R. Ettrich, H. Pelantová, M. Kuzma, S. Riva, D. Adámek, K. Bezouška a V. Křen. Charged hexosaminides as new substrates for b-N-acetylhexosami­nidase-catalyzed synthesis of immunomodulatory disaccharides. Adv. Synth. Catal. 2011, 353, 2409–2420.
  • C. Rech, R. R. Rosencrantz, K. Křenek, H. Pelantová, P. Bojarová, C. E. Römer, F. G. Hanisch, V. Křen a L. Elling. Combinatorial one-pot synthesis of poly-N-acetyllactosamine oligosaccharides with Leloir-glycosyltransfe­rases. Adv. Synth. Catal. 2011, 353, 2492–2500.
  • A. Drozdová, P. Bojarová, K. Křenek, L. Weignerová, B. Henssen, L. Elling, H. Christensen, H. H. Jensen, H. Pelantová, M. Kuzma, K. Bezouška, M. Krupová, D. Adámek, K. Slámová a V. Křen. Enzymatic synthesis of dimeric glycomimetic ligands of NK cell activation receptors. Carbohydr. Res. 2011, 346, 1599–1609.
  • K. Slámová, P. Bojarová, D. Gerstorferová, B. Fliedrová, J. Hofmeisterová, M. Fiala, P. Pompach a V. Křen. Sequencing, cloning and high-yield expression of a fungal β-N-acetylhexosami­nidase in Pichia pastoris. Prot. Express. Purif. 2012, 82, 212–217.
  • T. Desmet, W. Soetaert, P. Bojarová, V. Křen, L. Dijkhuizen, V. Eastwick-Field a A. Schiller: Enzymatic glycosylation of small molecules. Challenging substrates require tailored catalysts. Chem. Eur. J. 2012, 18, 10786–10801.
  • H. Mrázek, L. Weignerová, P. Bojarová, P. Novák, O. Vaněk a K. Bezouška. Carbohydrate synthesis and biosynthesis technologies for cracking of the glycan code: recent advances. Biotechnol. Adv. 2013, 31, 17–37.
  • P. Bojarová, R. R. Rosencrantz, L. Elling a V. Křen. Enzymatic glycosylation of multivalent scaffolds. Chem. Soc. Rev. 2013, 42, 4774–4797.
  • J. Krejzová, P. Šimon, Ľ. Kalachová, N. Kulik, P. Bojarová, P. Marhol, H. Pelantová, J. Cvačka, R. Ettrich, K. Slámová a V. Křen. Inhibition of GlcNAc-processing glycosidases by C-6-azido-NAG-thiazoline and its derivatives. Molecules 2014, 19, 3471–3488.
  • A. Šimonová, C. E. Kupper, S. Böcker, A. Müller, K. Hofbauerová, H. Pelantová, L. Elling, V. Křen a P. Bojarová. Chemo-enzymatic synthesis of LacdiNAc dimers of varying length as novel galectin ligands. J. Mol. Catal. B: Enzym. 2014, 101, 47–55.
  • P. Bojarová a V. Křen. Sugared biomaterial binding lectins: achievements and perspectives. Biomater. Sci. 2016, 4, 1142–1160.
  • K. Slámová a P. Bojarová. Engineered N-acetylhexosamine-active enzymes in glycoscience. Biochim. Biophys. Acta Gen. Subj. 2017, 1861, 2070–2087.
  • P. Bojarová, P. Chytil, B. Mikulová, L. Bumba, R. Konefał, H. Pelantová, J. Krejzová, K. Slámová, L. Petrásková, L. Kotrchová, J. Cvačka, T. Etrych a V. Křen. Glycan-decorated HPMA copolymers as high-affinity lectin ligands. Polym. Chem. 2017, 8, 2647–2658.
  • D. Laaf, P. Bojarová, B. Mikulová, H. Pelantová, V. Křen a L. Elling. Two-step enzymatic synthesis of b-D-N-acetylgalactosamine-(1→4)-D-N-acetylglucosamine (LacdiNAc) chitooligomers for deciphering galectin binding behavior. Adv. Synth. Catal. 2017, 359, 2101–2108.
  • D. Laaf, P. Bojarová, H. Pelantová, V. Křen a L. Elling. Tailored multivalent neo-glycoproteins: synthesis, evaluation, and application of a library of galectin-3-binding glycan ligands. Bioconjug. Chem. 2017, 28, 2832–2840.
  • D. Laaf, H. Steffens, H. Pelantová, P. Bojarová, V. Křen a L. Elling. Chemo-enzymatic synthesis of branched N-acetyllactosamine glycan oligomers for galectin-3 inhibition. Adv. Synth. Catal. 2017, 359, 4015–4024.
  • L. Bumba, D. Laaf, V. Spiwok, L. Elling, V. Křen a P. Bojarová. Poly-N-acetyllactosamine neo-glycoproteins as nanomolar ligands of human galectin-3: binding kinetics and modeling. Int. J. Mol. Sci. 2018, 19, 372.
  • P. Bojarová, M. R. Tavares, D. Laaf, L. Bumba, L. Petrásková, R. Konefał, M. Bláhová, H. Pelantová, L. Elling, T. Etrych, P. Chytil a V. Křen. Biocompatible glyconanomaterials based on HPMA-copolymer for specific targeting of galectin-3. J. Nanobiotechnol. 2018, 16, 73.
  • D. Laaf, P. Bojarová, L. Elling a V. Křen. Galectin-carbohydrate interactions in biomedicine and biotechnology. Trends Biotechnol. 2019, 37, 402–415.
  • P. Bojarová, N. Kulik, M. Hovorková, K. Slámová, H. Pelantová a V. Křen. The b-N-acetylhexosami­nidase in the synthesis of bioactive glycans: protein and reaction engineering. Molecules 2019, 24, 599.
  • P. Bojarová, N. Kulik, K. Slámová, M. Hubálek, M. Kotik, J. Cvačka, H. Pelantová a V. Křen. Selective β-N-acetylhexosami­nidase from Aspergillus versicolor – a tool for producing bioactive carbohydrates. Appl. Microbiol. Biotechnol. 2019, 103, 1737–1753.
  • J. Kapešová, L. Petrásková, K. Markošová, M. Rebroš, M. Kotik, P. Bojarová a V. Křen. Bioproduction of quercetin and rutinose catalyzed by rutinosidase: novel concept of “solid state biocatalysis”. Int. J. Mol. Sci. 2019, 20, 1112.
  • I. Bassanini, J. Kapešová, L. Petrásková, H. Pelantová, K. Markošová, M. Rebroš, K. Valentová, M. Kotik, K. Káňová, P. Bojarová, J. Cvačka, L. Turková, E. E. Ferrandi, I. Bayout, S. Riva a V. Křen. Glycosidase-catalyzed synthesis of glycosyl esters and phenolic glycosides of aromatic acids. Adv. Synth. Catal. 2019, 361, 2627–2637.
  • P. Bojarová, J. Bruthans a V. Křen. β-N-Acetylhexosami­nidases – the wizards of glycosylation. Appl. Microbiol. Biotechnol. 2019, 103, 7869–7881.

Running grant projects

  • LTC17005 Multivalentní glykopolymery jako ligandy biologických struktur s lektinovou prezentací.
  • LTC18038 Multivalentní glyko-biomateriály s potenciálem v regenerační medicíně.
  • LTC19038 Cílené glyko-nanomateriály pro diagnostiku a terapii rakoviny.

Teaching

Faculty of Biomedical Engineering, Czech Technical University in Prague (FBMI ČVUT):

  • Environmentální bezpečnost, prezenční forma (17PBPENV)
  • Environmentální bezpečnost, kombinovaná forma (17KBP2ENB)
  • Obecné laboratorní dovednosti (17PBLOLD)

Participating also in:

  • Základy patologie, hygieny a epidemiologie (17PBBZPD)
  • Fundamentals of Pathophysiology & Diagnostic Methods (17AMBZPD)
  • Základy klinických oborů I (17KBIKO1)

Department of Biochemistry, Faculty of Science, Charles university (PřF UK)

  • Biochemie a biomedicína sacharidů (MC250P64)
  • Pokročilé praktikum II (MC250C08)

My core team

Organic synthesis: Mgr. David Vrbata, Ing. Dorota Konvalinková

Biochemistry, molecular biology: Mgr. Pavlína Nekvasilová, Bc. Michaela Hovorková, Jakub Červený

Biology: Ing. Miluše Vlachová, PhD.

Biomaterials: Ing. Tereza Koukalová

Past team members (last 5 years): Mgr. Tomáš Vašíček, Bc. Lieselotte Sedláková, Bc. Iveta Andreasová, Bc. Nikola Rychlá, Mgr. Veronika Libová, RNDr. Barbora Mikulová

What´s up

  • I am a Member of the Scientific and Organizational Committees of Biotech 2020, chairing the Section on Industrial Biotechnology 
  • I am Assistant Editor of the journal Folia Microbiologica (Springer)
  • I was awarded Invited lecture at 30th International Carbohydrate Symposium (Shanghai, July 2020)
  • We welcome students at all levels to join us for their Bachelor, Master or Dissertation Thesis

Snapshots from the life of our team

Last update: 29/03/2020