- Start of Action – 18/04/2008; End of Action – 17/04/2012
Cascade Chemoenzymatic Processes – New Synergies Between Chemistry and Biochemistry
The main objective of the Action is to increase the innovative potential of biocatalysis by promoting the multistep catalytic concept, thus bringing this methodology closer to the application sphere.
The integration of biocatalysis into chemical processes is one of the three pillars (the pillar) of the European Platform on „Sustainable Chemistry“. This methodology also belongs to the fundamental strategies of „Green Chemistry“.
Therefore, this COST Action (acronym ) aims to enhance the synthetic potential of biocatalysis by coupling biotransformations one to another and integrating them with chemical steps to increase the product purity and yields close to quantitative values. A multidisciplinary approach is required to achieve this goal, involving precursor synthesis, enzyme screening, heterologous protein expression, enzyme mutagenesis, biocatalyst (co)-immobilization, bioreactor design and chiral product analysis.
CASCAT will consist of four Working Groups applying the multi-step approach to the synthesis/modification and transfer of glycosyl donors/acceptors, the deracemization of amino acids by multiple hydrolase systems, the synthesis of valuable amides and carboxylic acids from cheap chemicals (e.g. aldehydes) via combined action of enzymes involved in nitrile metabolism and entry or follow-up (chemo)enzymatic steps, the development of novel paths to biologically relevant chiral compounds using multienzyme-carboligation reactions or in situ cofactor regeneration.
This research will focus on the production of bioactive target molecules (e.g., non-natural amino-acids, glycoconjugates and mimetics, flavonoids, steroids) and chiral precursors of enantiopure pharmaceuticals.
Keywords: Cascade reactions, Multistep enzymatic transformations, Biocatalysis, Industrial Biotechnology, Sustainable Chemistry.
Domain: Chemistry and Molecular Sciences and Technologies
Action title: Cascade Chemoenzymatic Processes – New Synergies Between Chemistry and Biochemistry
WG3 Meeting in Prague – 16th May 2009
COST Action CM0701 „CASCAT“ meeting, Cascade Chemoenzymatic ProcessesNew Synergies Between Chemistry and Biochemistry, Meeting of the WG3, Cascade reactions on the nitrile group.
|9.15 – 9.25||Vladimír Křen (Head of the Department of Biogenesis and Biotechnology of Natural Compounds, Inst. Microbiol., Prague, CZ)|
|9.25 – 9.30||Ludmila Martínková (Inst. Microbiol., Prague, CZ)|
|Session I….. Chair: Laura Cantarella (Univ. Cassino, IT)|
|9.30 – 10.00||Margit Winkler (TU Graz – IMBT, AT)|
|“NITRILASE VARIANT WITH ENHANCED STABILITY AT ACIDIC CONDITIONS”|
|10.00 – 10.30||Giovanni Gambera (CNR Catania, IT)|
|“PERSPECTIVES IN THE SYNTHESIS OF NOVEL BIOMEDICAL VALUED NITRILE DERIVATIVES AND THEIR BIOTRANSFORMATIONS”|
|10.30 – 11.15||Coffee break|
|Session II….. Chair: Linda Otten (TU Delft, NL)|
|11.15 – 11.45||Karel Bezouška (Invited expert; Charles University, Prague, CZ)|
|“FOLDING OF FUNCTIONALLY ACTIVE PROTEINS IN VITRO: NEW POSSIBILITIES AND NEW PROCEDURES”|
|11.45 – 12.15||Olga Sosedov (Univ. Stuttgart, DE)|
|“AMIDE SYNTHESIS VIA NITRILASE FROM PSEUDOMONAS FLUORESCENS EBC 191”|
|12.15 – 12.45||Ondřej Kaplan (Inst. Microbiol., Prague, CZ)|
|“HETEROLOGOUS EXPRESSION OF FUNGAL NITRILASES”|
|12.45 – 14.00||Lunch|
|Session III….. Chair: Nicola D´Antona (CNR Catania, IT)|
|14.00 – 14.30||Miroslav Pátek (Inst. Microbiol., Prague, CZ)|
|“ANALYSIS OF NITRILE HYDRATASE-AMIDASE OPERON FROM RHODOCOCCUS ERYTHROPOLIS”|
|14.30 – 15.00||Maria Cantarella (Univ. L´Aquila, IT)|
|“USE OF NITRILE HYDRATASE-AMIDASE CASCADE SYSTEM FOR NITRILE BIOCONVERSION”|
|15.00 – 15.30||Sander van Pelt (TU Delft, NL)|
|“NITRILE HYDRATASES IN ORGANIC SYNTHESIS”|
|15.30 – 16.00||Linda G. Otten (TU Delft, NL)|
|“CLONING AND EXPRESSION OF NEW NITRILE HYDRATASES”|
|16.00 – 16.45||Coffee break; Discussions in groups|