Ing. Ludmila Martínková, CSc. DSc.

Position:
Senior Scientist
Email:
martinko@biomed.cas.cz
Phone:
(+420) 296 442 569
Address:
Laboratory of Biotransformation
Institute of Microbiology of the Czech Academy of Sciences
Vídeňská 1083
Praha 4
142 20
Czech Republic

Publications

  • 78 articles in peer-reviewed journals (Web of Science)
  • 9 book chapters
  • citation index (with autocitations): 2905 (Web of Science)
  • h-index: 29 (Web of Science)

ORCID: 0000-0002-1937-7081

Research interests

  • Nitrilases, cyanide hydratases
  • Aldoxime dehydratases
  • Tyrosinases, laccases
  • Genome mining, overproduction of enzymes
  • Biotransformation (aldoximes, nitriles, phenols)
  • Biodegradation (cyanides,nitriles, phenols)
  • Valorization of lignin monomers
  • Product analysis (HPLC)

Education

  • Institute of Chemical Technology, Prague (Fermentation Technology and Bioengineering) – MSc (1984)

Scientific degrees

  • Czechoslovak Academy of Sciences, Institute of Microbiology – PhD in biology/micro­biology (1989)
  • Czech Academy of Sciences – DSc. (= Res. Prof.) award (2018) – Thesis “Microbial Enzyme Transformations of Cyano and Phenolic Compounds: Advances, Challenges and Perspectives”

International experience

  • 1990 (1 month) Institute of Biochemistry, TU Wien (FEMS Fellowship)
  • 1995, 1997 (total 3 months) Institute of Microbiology, University of Stuttgart (supported by DAAD Fellowship and Univ. Stuttgart)
  • 1999-2000 short-term stays at Institute of Organic Chemistry, TU Graz, Institute of Organic Chemistry, University of Debrecen, Institute of Organic Chemistry, and University of Hamburg
  • selected international collaborations: Dr. Margit Winkler, Dr. Norbert Klempier, TU Graz, Austria; Prof. Florian Rudroff, TU Wien, Austria; Prof. Jean Bolte, University of Clermont-Ferrand, France; Prof. Maria Cantarella, University of L´Aquila, Italy; Prof. Herfried Griengl, TU Graz, Austria; Prof. Lutz Fischer, University of Hohenheim – Stuttgart, Germany; Dr. Giovanni Nicolosi, CNR, Catania, Italy; Prof. Marius Réglier, University of Aix Marseille, France; Dr. Andreas Stolz, University of Stuttgart, Germany
  • COST Management Committees Member in Actions D25, CM0701, CM1003 and CA17128; WG coordinator in Action D25

Selected recent projects

  • 2020–2022 CSF – FWF (Austria) – Lead Agency Project GF20-23532L  A new chemoenzymatic route from carboxylic acids to nitriles. Team member, working group coordinator.
  • 2019–2022 MEYS, CZ – LTC19037 Valorization of lignin using fungal oxidases. Project coordinator.
  • 2018–2020 MEYS, CZ – LTC18080 Functionalization of C-H bonds in phenol compounds by tyrosinases. Project coordinator.
  • 2018–2020 Czech Science Foundation (CSF), GA18-00184S New nitrilase superfamily proteins in Basidiomycota. Project coordinator.
  • 2014–2017 Agency of Technology, CZ – TA04021212 Development of technology for removal of organic substances from coking plant sewage. Coordinator.

Selected recent publications

  • Dominic Goj, Stella Ebner, Melissa Horvat, Simon Arhar, Ludmila Martínková, Margit Winkler: Cell-free reduction of carboxylic acids with secreted carboxylic acid reductase. Journal of Biotechnology, 382 (February 2024), 44-50 (2024). IF 4,1.
  • Ludmila Martínková, Michal Grulich, Miroslav Pátek, Barbora Křístková, Margit Winkler: Bio-Based Valorization of Lignin-Derived Phenolic Compounds: A Review. Biomolecules, 13 (5), 717: 1-25 (2023). IF 6,064.
  • Ludmila Martínková, Natalia Kulik, Anastasia Sedova, Barbora Křístková, Pavla Bojarová: Recent Progress in the Production of Cyanide-Converting Nitrilases-Comparison with Nitrile-Hydrolyzing Enzymes. Catalysts, 13 (3), 500:1-19 (2023). IF 4,501.
  • Margit Winkler, Horvat Melissa, Schiefer Astrid, Weilch Victoria, Rudroff Florian, Pátek Miroslav, Martínková Ludmila: Organic Acid to Nitrile: A Chemoenzymatic Three-Step Route. Advanced Synthesis & Catalysis, 365 (1), 37-42 (2023). IF 5,981.
  • Ludmila Martínková, Pavla Bojarová, Anastasia Sedova, Vladimír Křen: Recent trends in the treatment of cyanide-containing effluents: Comparison of different approaches. Critical reviews in environmental science and technology, 53 (3), 416-434 (2023). IF 11,750.
  • Barbora Křístková, Robert Rädisch, Natalia Kulik, Melissa Horvat, Lenka Rucká, Milan Grulich, Florian Rudroff, Alan Kádek, Miroslav Pátek, Margit Winkler, Ludmila Martínková: Scanning aldoxime dehydratase sequence space and characterization of a new aldoxime dehydratase from Fusarium Vanettenii. Enzyme and Microbial Technology, 164, 110187:1-13 (2023). IF 3,705.
  • Ludmila Martínková, Barbora Křístková, Vladimír Křen: Laccases and Tyrosinases in Organic Synthesis. International Journal of Molecular Sciences, 23 (7), 3462:1-19 (2022).  IF 6,208.
  • Robert Rädisch, Miroslav Pátek, Barbora Křístková, Margit Winkler, Vladimír Křen, Ludmila Martínková: Metabolism of Aldoximes and Nitriles in Plant-Associated Bacteria and Its Potential in Plant-Bacteria Interactions. Microorganisms, 10 (3), 549:1-16 (2022).  IF 4,926.
  • Melissa Horvat, Victoria Weilch, Robert Rädisch, Sebastian Hecko, Astrid Schiefer, Florian Rudroff, Birgit Wilding, Norbert Klempier, Miroslav Pátek, Ludmila Martínková, Margit Winkler: Chemoenzymatic one-pot reaction from carboxylic acid to nitrile via oxime†.
    Catalysis Science & Technology, 12 (1), 62-66 (2022). IF 6,177.
  • Sedova A., Rucká L., Bojarová P., Glozlová M., Novotný P., Křístková B., Pátek M., Martínková L.*: Application Potential of Cyanide Hydratase from Exidia Glandulosa: Free Cyanide Removal from Simulated Industrial Effluents. Catalysts, 11 (11), 1410:1-16 (2021). IF 4,146.
  • Rucká L., Kulik N., Novotný P., Sedova A., Petrásková L., Příhodová R., Křístková B., Halada P., Pátek M., Martínková L.*: Plant nitrilase homologues in fungi: Phylogenetic and functional analysis with focus on vitrilases in Trametes versicolor and Agaricus bisporus. Molecules 25, art. no. 3861 (2020). IF 3,060.
  • Martínková L., Příhodová R., Kulik N., Pelantová H., Křístková B., Petrásková L., Biedermann D.: Biocatalyzed reactions towards functional food components 4-alkylcatechols and their analogues. Catalysts 10, art. no. 1077 (2020). IF 4,146.
  • Martínková L. *: Nitrile metabolism in fungi: A review of its key enzymes nitrilases with focus on their biotechnological impact.* Fung. Biol. Rev. 33, 149-157 (2019). IF 5.563
  • Rucká L., Chmátal M., Kulik N., Petrásková L., Pelantová H., Novotný P., Příhodová R., Pátek M., Martínková L.*: Genetic and functional diversity of nitrilases in Agaricomycotina. Inst. J. Mol. Sci. 20, art. no. 5990 (2019). IF 4.784
  • Rädisch R., Chmátal M., Rucká L., Novotný P., Petrásková L, .Halada P., Kotik, M., Pátek, M., Martínková L.*: Overproduction and characterization of the first enzyme of a new aldoxime dehydratase family in Bradyrhizobium sp. Int. J. Sci. Macromol. 115, 746-753 (2018). IF 4,784
  • Martínková L., Křen V.: Biocatalytic production of mandelic acid and analogues: a review and comparison with chemical processes. Applied Microbiology and Biotechnology, 102 (9), 3893-3900 (2018). IF 3,340.
  • Martínková L.*, Kotik M., Marková E., Homolka L.: Biodegradation of phenolic compounds by Basidiomycota and its phenol oxidases: A review. Chemosphere 149, 373–382 (2016). IF (2016) 3.340
  • Marková E., Kotik M., Křenková A., Man P., Haudecoeur R., Boumendjel A., Hardré R., Mekmouche Y., Courvoisier-Dezord E., Réglier M., Martínková L.*: Recombinant tyrosinase from Polyporus arcularius: Overproduction in Escherichia coli, characterization, and use in a study of aurones as tyrosinase effectors. J. Agric. Food Chem. 64, 2925−2931 (2016). IF (2016) 2.912
  • Martínková L.*, Chmátal M.: The integration of cyanide hydratase and tyrosinase catalysts enables effective degradation of cyanide and phenol in coking wastewaters. Wat. Res. 102, 90–95 (2016). IF (2016) 7.913

*corresponding author

Pedagogical activities

  • Supervision of students who obtained PhD (8)
  • Currently supervisor of one PhD student
  • Reviews of PhD theses (Charles Univ. in Prague, Univ. of Sydney, Univ. of Cape Town)
  • member of the Postgraduate Program Biochemistry and Bioorganic Chemistry (ICT)

Research organization activities

  • Evaluator in Horizon 2020 (2016 – 2020)
  • Evaluator in Horizon Europe (2021)
  • COST MC Member in Actions D25, CM0701 and CM1003
  • Member of symposium organization committees (Biotrans 2003 Olomouc, Biocat 2006 Hamburg)
  • Guest Editor – Special Issue „Nitrilases and Nitrile Hydratases“ (Molecules, 2020) – with Dr. Margit Winkler (TU Graz)
  • Member of Editorial Boards (Biotechnology Letters, Biocatalysis and Biotransformation)
  • Reviewer for journals Advanced Synthesis&Catalysis, Applied and Environmental Microbiology, Applied Microbiology and Biotechnology, Biotechnology Advances,  BMC Biotechnology, Ecology and Environmental Safety, Extremophiles, Journal of Agricultural and Food Chemistry, Molecules etc.

Membership

  • Czechoslovak Society for Microbiology
  • Czech Society for Biochemistry and Molecular Biology
  • Czech Chemical Society

Languages

  • Czech (mother tongue)
  • English, German (fluent)
  • French, Italian, Russian, Latin (basic)

 

Last update March 8, 2023