Ing. Miroslav Šíša, Ph.D.

Position:
Senior Scientist
Email:
miroslav.sisa@biomed.cas.cz
Phone:
(+420) 296 442 360
Address:
Laboratory of Biotransformation
Institute of Microbiology of the Czech Academy of Sciences
Vídeňská 1083
Praha 4 – Krč
142 00
Czech Republic

Education

  • 2005: Ph.D. in Organic chemistry at Charles University, Prague (Faculty of Science)
  • 1999: Ing. in Organic chemistry at University of Chemistry and Technology, Prague (Faculty of Chemical Technology)
  • 1993–1995: Charles University, Faculty of Natural Sciences, Prague, Czech Republic

Research Orientation

Organic chemistry and synthesis, medicinal chemistry, drug design, natural products

Employment

  • 1999 Max Planck Institute, Cologne, Germany  Ph.D. intership “Research on Brassinosteroids”
  • 1999–2005 Charles University, Faculty of Natural Sciences, elaborated in: Academy of Sciences of the Czech Republic, Institute of Organic Chemistry and Biochemistry, Department of Medicinal Steroids, Prague, Czech Republic
    Ph.D. in organic chemistry “New Brassinosteroids Analogues”, Advisor: RNDr. Ladislav Kohout, DrSc.
  • 2004–2005 IQS, University Ramon Llull, Barcelona, Spain
    Ph.D. intership “Brassinolide side chain simulation and molecular modeling explanation of SAR”
  • 2005–2006 Academy of Sciences of the Czech Republic, Institute of Organic Chemistry and Biochemistry, Department of Medicinal Steroids, Prague, Czech Republic
    Postdoctoral Research Assistant “SAR of brassinosteroids”
  • 2006–2008 CSIC, Department of Biologic and Organic Chemistry, Barcelona, Spain
    Postdoctoral Research Assistant “An approach to the design of chemical chaperones as new potential therapeutic agents”, Advisor: Prof. Dr. A. Delgado
  • 2008–2009 PCB-UB-IRBB, Department of Chemistry and Molecular Pharmacology, Barcelona, Spain
    Postdoctoral Research Assistant “Synthesis of natural Aplicyanins and analogues and evaluation of its anticancer biological activity”, Advisors: Prof. Dr. F. Albericio, Prof. Dr. M. Alvarez
  • 2009 Academy of Sciences of the Czech Republic, Palacky University, Olomouc, Czech Republic
    Postdoctoral Research Assistant “Fluorescent brassinosteroids labeling for biosynthesis studies”, Advisor: Prof. M. Strnad
  • 2010–2011 University of Free States, Department of Organic Chemistry, Bloemfontein, South Africa “The development of novel methodology to synthesize unavailable 3 and 4-coupled proanthocyanidins in sufficient quantities for bioassay testing and drug development“, Advisor: Prof Jan (H) Van der Westhuizen
  • 2011–2013 University of Basel, Switzerland “Lewis Acid Catalysed Inverse Electron-Demand Diels-Alder Reaction – A New Synthetic Entry to Steroids”, Advisor: Prof. H. A. Wegner
  • 2013-2014 Friedrich-Alexander-Universität Erlangen, Germany “Ferrocene based prodrugs” and “Chemiluminiscent DNA labeling”, Advisor: Prof. A. Mokhir
  • 2014-2024 Institute of Experimental Botany – ACSR, Czech Republic, “Synthesis of quinone derivatives as potential anti-inflammatory agents”, “Synthesis of Hh/SMO inhibitors as a potential anticancer drugs” and “COX-1 selective drugs as cardioprotective and antiplatelet targets”
  • 2024-2025University of Chemistry and Technology Prague, Department of Chemistry of Natural Compounds, Diagnostic and Therapeutic Nanosystems, currently working on “Synthesis of liposomal lipoglykopeptide adjuvants” as a part of REcombinant TEchnologies for MEDicine (RETEMED) project

Grants and Fellowships

  • 2005-2007 Junior Grant of the Agency of Academy of Sciences of the Czech Republic.
  • 2006-2008 Fellowship of the Ministry of Education and Sciences of Spain.
  • 2010-2011 Fellowship of the National Research Foundation of South Africa.
  • 2011-2013 Sciex Fellowship in Switzerland.
  • 2015-2017 Grant LD15006 from the Ministry of Education, Youth and Sports of the Czech Republic and networking contribution by the COST Action CM1407 “Challenging organic syntheses inspired by nature – from natural products to drug discovery”.
  • 2016-2018 Grant TG03010009 from Technology Agency of the Czech Republic “New COX-2 and 5-LOX inhibitors”.
  • 2018-2021 Grant LTC16225 from the Ministry of Education, Youth and Sports of the Czech Republic and networking contribution by the COST Action CA16225 “Realizing the therapeutic potential of novel cardioprotective therapies”.

Affiliations

Member of:

  • Czech Chemical Society.
  • COST Action CM1407 “Challenging organic syntheses inspired by nature – from natural products to drug discovery” as MC substitute.
  • COST Action CA16225 “Realizing the therapeutic potential of novel cardioprotective therapies” as MC substitute.
  • COST Action CA19138 “Lobular Breast Cancer: Discovery Science, Translational Goals, Clinical Impact (LOBSTERPOT)” as MC.
  • COST Action CA21111 “One Health drugs against parasitic vector borne diseases in Europe and beyond (OneHealthDrugs)”.
  • COST Action CA22109 “Medicinal plants for animal health care: Translating tradition into modern veterinary medicine (MedPlants4Vet)” as MC.
  • COST Action CA23156 “European Network for Sigma-1 Receptor as a Therapeutic Opportunity (SIGMA-1EUROPE)” as MC.

Languages

  • English, Spanish, German (beginner)

List of Publications

  • Simple NMR determination of 5α/5β configuration of 3-oxosteroids: Chodounská, H., Buděšínský, M., Šídová, R., Šíša, M., Kasal, A., Kohout, L.: Colect. Czech. Chem. Commun. 2001, 66, 1529–1544.
  • Synthesis of 7a homo and 7a,7b dihomo 5α-cholestane analogues of brassinolide: Šíša, M., Buděšínský, M., Kohout, L.: Colect. Czech. Chem. Commun. 2003, 68, 2171–2189.
  • Synthesis of new androstane brassinosteroids with 17β ester groups – butyrates, heptafluorobutyrates and laurates: Šíša, M., Hniličková, J., Swaczynová, J., Kohout, L.: Steroids 2005, 70(11), 755-162.
  • Synthesis, biological, immunological and anticancer properties of a new brassinosteroid ligand: Swaczynová, J., Šíša, M., Hniličková, J., Kohout , L., Strnad, M.: Pol. J. Chem. 2006, 80(4), 629-635.
  • New Techniques for the Estimation of Naturally Occurring Brassinosteroids: Swaczynová, J., Novák, O., Hauserová, E., Fuksová K., Šíša, M., Kohout, L., Strnad M.: J. Plant Growth Regul. 2007, 26(1).
  • Brassinolide activities of 2α,3α-diols versus 3α,4α-diols in the bean second internode bioassay: Explanation by molecular modeling methods: Šíša, M., Vilaplana-Polo, M., Brosa Ballesteros, C. And Kohout L.: Steroids 2007, 72(11-12), 740-750.
  • Small-Scale One-Pot Reductive Alkylation of Unprotected Aminocyclitols with Supported Reagents: Šíša, M., Trapero, A., Llebaria, A., Delgado, A.: Synthesis 2008, 19, 3167-3170.
  • Total Synthesis and Anti-tumor Activity Screening of (±)-Aplicyanins A, B and E and Related Analogs: Šíša, M., Pla, D., Altuna, M., Franchesch, A., Cuevas, C., Alvarez, M., Albericio, F.: J. Med. Chem. 2009, 52(20), 6217-6223.
  • Review: Photochemistry of Flavonoids: Šíša, M., Bonnet, S.L., Ferreaira, D., Van der Westhuizen, J.H.: Molecules, 2010, 15(8), 5196-5245.
  • Synthesis of C-3 Coupled Biflavonoids and C-3 Coupled Biflavonoids Analogues: Van der Westhuizen, J.H., Bonnet, S.L., Achilonu, M., Šíša, M. PCT Application number PCT/IB2010/053755 based on ZA Provisional South African Patent Application. 2009/05765, August 19, 2009.
  • Analysis of commercial proanthocyanidins. Part 1: The chemical composition of quebracho (Schinopsis lorentzii and Schinopsis balansae) heartwood extract: Venter, P. B., Šíša, M., Van der Merwe, J., Bonnet S. L., Van der Westhuizen, J.H.: Phytochemistry 2012, (95), 95-105.
  • Fluorescent castasterone reveals BRI1 signaling from the plasma membrane: Irani, N.G., Di Rubbo, S., Mylle, E., Pizon, J., Van den Begin, J., Hniličková, J., Šíša, M., Szatmári A-M., Blasi J.V., Van Damme, D., Kohout, L., Strnad M., Schumacher, K., Caño-Delgado, A.I., Madder, A., Friml, J., Russinova, E.: Nature Chem. Biol. 2012, 8(6):583-589.
  • Synthesis of Tetracyclic Diterpenoids with Pharmacologic Relevance: Sisa. M., Vanek, T.: Curr. Pharm. Des. 2016, 22(12): 1767-1807.
  • Metabolism of ibuprofen in higher plants: A model Arabidopsis thaliana cell suspension culture system: Marsik P., Sisa M., Lacina O., Motkova K., Langhansova L., Rezek J., Vanek T.: Enviromental Pollution 2017, 220, 383-392.
  • Concise access to primin, miconidin and related natural resorcinols: Sisa M., Dvorakova M., Vanek T: Tetrahedron 2017, 73(35), 5297-5301.
  • Primin derivatives, mtehods of preparation thereof and use therof: Landa P., Sisa M., Vanek T., Rarova L., WO 2017/036436 A1, PCT/CZ2016/050033
  • Lysosome-targeting amplifiers of reactive oxygen species as anticancer prodrugs: Daum S., Reshetnikov V., Sisa M., Dymych T., Lootsik M.D., Bilyy R., Bila E., Janko C., Alexiou C., Hermann A., Selnner L., Mokhir A.: Angew. Chem. Int. Ed. 2017, 56(49), 15545-15549.
  • Synthesis, inhibitory activity and in silico docking of dual COX/5-LOX inhibitors with quinone and resorcinol core: Sisa M., Dvorakova M., Temml V., Jarosova V., Vanek T., Landa p.: Eur. J. Med. Chem.2020, 204, 112620-112630.
  • An Endoplasmic Reticulum Specific Pro-amplifier of Reactive Oxygen Species in Cancer Cells: Xu HG., Schikora M., Šíša M., Daum S., Klemt I., Janko C., Alexiou C., Bila G., Bilyy R., Gong WJ., Schmitt M., Sellner L., Mokhir A: Angew. Chem. Int. Ed. 2021, 60(20), 11158-11162.
  • Fate of antifungal drugs climbazole and fluconazole in constructed wetlands-Diastereoselective transformation indicates process conditions: Sochacki A., Maršík P., Chen ZB., Šíša M.,Vymazal J.: Chem. Eng. J. 2021, 421, 127783-127791.
  • S-560 and mofezolac isosteres as new potent COX-1 selective inhibitors with antiplatelet effect: Sisa M., Konečný L., Temml V., Carazo A., Mladěnka P., Landa P. Arch. Pharm. 2023, 356, e2200549.

 

Last update: February 12, 2026