Prof. Ing. Vladimír Křen, PhD., DSc., FRSC

Position:
Senior Research Fellow, Founder of the Laboratory of Biotransformation in 1994 and its head 1994-2021
Email:
kren@biomed.cas.cz
Phone:
(+420) 296 442 510
Address:
Laboratory of Biotransformation
Institute of Microbiology of the Czech Academy of Sciences
Vídeňská 1083
Praha 4 – Krč
142 20
Czech Republic

Education

  • Graduated with honours at the Institute of Chemical Technology in Prague in Fermentation chemistry and biotechnology – degree: Diploma-Engineer (Dipl.-Ing.), 1981.
  • CSc. (Candidatus Scientiarum – equal to PhD) in Biology (area microbiology) from Cz. Academy of Sciences, Institute of Microbiology, Prague in 1988.
  • DSc (Doctor Scientiarum) in Biology from Academy of Sciences of the Czech Rep., Prague 2001.
  • Habilitation (docent) in Organic chemistry, Palacky University, Olomouc, 2001.
  • Professor (full) in Medicinal chemistry, Palacky University, Olomouc, 2005.

Employment

  • 1981 – 1988 Research assistant, Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague
  • 1988 Research fellow in Lab. of Metabolites of Eukaryotic Microorganisms, Inst. Microbiol.
  • 1993 Head of Laboratory of Biotransformation, Inst. Microbiol. Prague (current position).
  • 2001 Head of Department of Biotechnology of Natural Products (2001-2003; 2007-2013).

Scientific Orientation

  • Secondary metabolites of fungi (Aspergillus, Claviceps); Immobilised microbial cells; Biotransformation of natural products by enzymes and microorganisms.
  • Glycosidases and glycosyltransferases, pharmacology of glycosides, glycobiology.
  • Flavonoids, antioxidants.

Fellowships stays

  • 1979 Alko Co., Koskenkorva, Helsinki, Finland (3 months). Program: Undergraduate student
    training – kinetics of yeast fermentation
  • 1985 E.-M. Arndt University, Greifswald (Prof. F. Bottcher), and Institute of Plant
    Physiology, Halle a. Saale (Prof. D. Groger), Germany (3 months); Program: Fungal protoplasts,
    preparation and fusion, mutasynthesis of ergot alkaloids.
  • 1990 – 1992 University of Warwick, U.K. Prof. D.H.G. Crout (15 months); Program:
    Enzymatic biotransformations, production of optically active compounds, enzymatic glycosylations,
    teaching.
  • 1993 Université Paris-Sud, Orsay, France (3 months) Dr. C. Augé; Program: Enzymatic
    biotransformation of ergot alkaloids
  • 1993 – 1994 University of Hamburg (A. von Humboldt fellow) Prof. J. Thiem (8 months);
    Program: Purification of glycosyltransfe­rases and their use for biotransformation of ergot
    alkaloids and other natural substances.´
  • 1994 University of Bologna (3 months) Prof. A. Minghetti; Program: Biotransformations of
    natural products by plant cell cultures.
  • 1996 University of Okayama, Kurashiki, Japan (3 months) Matsumae Foundation, visiting
    professor

Membership in Scientific Societies

  • Czech Society of Chemistry
  • Czechoslovak Society of Microbiology
  • British Royal Society of Chemistry (FRSC)
  • American Chemical Society

Membership in Editorial Boards

  • Journal of Carbohydrate Chemistry
  • Folia Microbiologica
  • Internation Journal of Molecular Sciences
  • International Journal of Carbohydrate Chemistry

Scientific-organizatory activities

  • Head of laboratory (15 research workers & PhD students) – date
  • Head of department: 2001–2003, 2007–2013
  • Vice-chairman of Scientific board of the Institute of Microbiology, Academy of Sciences
    1996–2001.
  • Member of Scientific board of the Inst. of Organic Chemistry and Biochemistry, Acad. Sci.
    1992–2001
  • Chairman of Chemistry section of the Grant Agency of the Cz. Acad. Sci. 1995–98
  • Editorial board of Journal of Carbohydrate Chemistry – member
  • Representative of the Czech Republic in EUROCARB (European Carbohydrate Organization)
  • Representative of the Czech Republic in ICO (International Carbohydrate Organization)

Project coordination responsibility as PI

  • Industrial grant – „Glycosylation of pharmaceuticals“ 1994, 1995
  • EU Grant PECO Preparation of ergot alkaloid O- and N- glycosides with antiviral activities.
  • EU Grant PECO Glycosylation of flavonoids with potential hepatoprotective activities.
  • Volkswagen Foundation reserach grant.
  • Grant of Czech Science Foundation – 17 grants up to now
  • Grant of Czech Ministry of Industry – 3 grants up to now
  • Grant of Czech Ministry of Education – 11 grants up to now
  • Grant of Czech Ministry of Health – 2 grants up to now
  • Grant of Czech Academy of Sciences – 5 grants up to now
  • Grant of British Royal Society of Chemistry (3x)
  • National Center for Biotransformation and Biocatalysis (coordinator)
  • 2 EU Grants FP7 (KBBE-2010-4-266025 BIONEXGEN Developing the next generation of biocatalysts for iIndustrial chemical synthesis; KBBE.2010.3.3 265854 NOVOSIDES Novel biocatalysts for the production of glycosides)
  • Bilateral international research projects with Italy (3x), USA (2x), GB, Argentina, Japan, Germany (2x)
  • Member of Management committee of EU ESF COST actions Nos. D25, D34, CM0602, CM0701, CM0804, CM1001, CM1102, BM1203, CM1303, CM1407, CA15136, CA18132, CA18229

Expert Activity for the Czech Government

  • Ministry of Industry and Commerce – Member of ministerial advisory board for industrial
    grants
  • Ministry of Education – Member of the ministerial advisory board for young scientists
    returning from foreign stays
  • Ministry of Education – Member of the ministerial advisory board for institutional grants
  • Ministry of Education – Chairman of the ministerial board for the Inter-COST grants
  • Czech Government – Member of governmental advisory board for science evaluation

Expert Activity for EU

  • Evaluation of grant applications of 5th and 6th and 7th FW – Food quality and
    microbiology
  • Evaluation of applications for Marie Curie training centers and Centers of Excellence CERC
  • Evaluation of H2020 projects
  • Preparation of the position materials for EU (SusChem)
  • Evaluation of the IMI projects

Participation in COST activities

  • MC member of Cost Chemistry D25, D28, D34, CM0601, CM0702, CM1001, CM1102, BM1203, CA15136,
    CA16225
  • Coordinator of WG D25–0001–02, WG-MC0702–1, WG-CM1001
  • Participant in WG D13–0001–98 and WG D13–0015–01, WG
    D28–0007–03, WG D34–0001–05, MC0601
  • Organizer of Workshop of COST D25, June 28, 2003 Olomouc

Research Awards

  • Price of rector for outstaning study results (1981)
  • Distinguished young scientist achievement medal – from Czech Academy of Sciences (1988)
  • A. v Humboldt research fellowship (1993)
  • Matsumae International Foundation Research Award, Japan (1995-6)
  • Invited lecture – Gordon conference “Biocatalysis”, Boston, USA (1996); Gordon conference “Carbohydrates”, Boston, USA (2017)
  • Czech Academy of Sciences Award for Excellent project results (2003)
  • Hanus Medal from the Czech Chemical Society (2016)

Hobbies

  • Biking
  • Swimming
  • Scuba-diving

Language capabilities 

  • English – fluent
  • German – working knowledge
  • French – working knowledge
  •  Russian – working knowledge
  •  Slovak – fluent

Publications

  • 350 papers in peer rewieved journals and book chapters (80 papers in last 5 years)
  • 8130 citations (6245 without self-citations)
  • 23 patents
  • Hirsch index: 42

List of selected publications relevant to the project – last 5 years (and main collaborative papers with prof. Elling)

  1. Bojarová, R. R. Rosencrantz, L. Elling, V. Křen: Enzymatic glycosylation of multivalent scaffolds. Chem. Soc. Rev. 42, 4774 (2013). IF 30.425
  2. Šimčíková, M. Kotik, L. Weignerová, P. Halada, H. Pelantová, K. Adamcová, V. Křen: α-L-Rhamnosyl-β-D-glucosidase (rutinosidase) from Aspergillus niger: Characterization and synthetic potential of a novel diglycosidase. Adv. Synth. Catal. 357, 107 (2015). IF 6.453.
  3. Slámová, J. Krejzová, P. Marhol, L. Kalachova, N. Kulik, H. Pelantová, J. Cvačka, V. Křen: Synthesis of derivatized chitooligomers using transglycosidases engineered from the fungal GH20 β-N-Acetylhexosaminidase. Adv. Synth. Catal. 357, 1941 (2015) IF 6.453.
  4. Gavezzotti, F. Bertacchi, G. Fronza, V. Křen, D. Monti, S. Riva: Laccase-catalyzed dimerization of piceid, a resve-ratrol glucoside, and its further enzymatic elaboration. Adv. Synth. Catal. 357, 1831 (2015). IF 6.453.
  5. Lange, A. Šimonová, T. Fischöder, H. Pelantová, V. Křen, L. Elling: Towards keratan sulfate – Chemo-enzymatic cascade synthesis of sulfated N-acetyllactosamine glycan oligomers. Adv. Synth. Catal. 358, 584 (2016). IF 6.453
  6. Bojarová, V. Křen: Sugared biomaterials binding lectins: from in vivo imaging to cancer therapy. Biomaterials Science 4, 1142-1160 (2016). IF 3.614
  7. Bassanini, J. Krejzová, W. Panzeri, V. Křen, D. Monti, S. Riva: A sustainable one-pot two-enzyme synthesis of naturally occurring arylalkyl glucosides. ChemSusChem 10, 2040 (2017). IF 7.226
  8. Bojarová, P. Chytil, B. Mikulová, L. Bumba, R. Konefał, J. Krejzová, K. Slámová, L. Petrásková, L. Kotrchová, H. Pelantová, J. Cvačka, T. Etrych, V. Křen: Glycan-decorated HPMA copolymers as high-affinity lectin ligands. Polymer Chemistry 8, 2647 (2017). IF 5.375
  9. Laaf, P. Bojarová, B. Mikulová, H. Pelantová, V. Křen, L. Elling: Two-step enzymatic synthesis of β-D-N-acetylgalactosamine-(1®4)-D-N-acetylglucosamine (LacdiNAc) chitooligomers for deciphering galectin binding behavior. Adv. Synth. Catal. 359, 2101 (2017). IF 5.646
  10. Laaf, H. Steffens, H. Pelantová, P. Bojarová, V. Křen, L. Elling: Chemo-enzymatic synthesis of branched N-acetyllactosamine glycan oligomers for galectin-3 inhibition. Adv. Synth. Catal. 359, 4015 (2017). IF 5.646
  11. Laaf, P. Bojarová, H. Pelantová, M. Bienert, C. Schmitz, C. Dey, T. Fischöder, S. Neuss-Stein, W. Jahnen-Dechent, V. Křen, L. Elling: Tailored multivalent neo-glycoproteins: Synthesis, evaluation, and application of a library of galectin-3-binding glycan ligands. Bioconjugate Chemistry 28, 2832 (2017). IF 4.818
  12. L. Bumba, D. Laaf, V. Spiwok, L. Elling, V. Křen, P. Bojarová: Poly-N-acetyllactosamine neo-glycoproteins as nanomolar ligands of human galectin-3: Binding Kinetics and Modeling. Int. J. Mol. Sci., 19, No. 372 (2018). IF 3.226
  13. P. D. Bojarová, M. R. Tavares, D. Laaf, L. Bumba, L. Petrásková, R. Konefał, M. Bláhová, H. Pelantová, L. Elling, T. Etrych, P. Chytil, V. Křen: Biocompatible glyconanomaterials based on HPMA-copolymer for specific targeting of galectin-3. Journal of Nanobiotechnology 16:73 (2018). IF 5.294
  14. D. Laaf, P. Bojarová, L. Elling, V. Křen: Galectin-carbohydrate interactions in biomedicine and biotechnology. Trends in Biotechnol. 37, 402-415 (2019). IF 13.578
  15. V. Heine, S. Boesveld, H. Pelantová, V. Křen, C. Trautwein, P. Strnad, L. Elling: Identifying efficient Clostridium difficile toxin A binders with a multivalent neo-glycoprotein glycan library. Bioconjugate Chemistry 30, 2373-2383 (2019). IF 4.349
  16. R. Tavares, M. Bláhová, L. Sedláková, L. Elling, H. Pelantová, R. Konefał, T. Etrych, V. Křen, P. Bojarová, P. Chytil: High-affinity HPMA copolymers with tailored N-acetyllactosamine presentation discriminate between galectins. Biomacromolecules, 21, 641-652 (2020). IF 6,092
  17. M. Filipová, P. Bojarová, M.R. Tavares, L. Bumba, L. Elling, P. Chytil, K. Gunár, V. Křen, T. Etrych, O. Janoušková: Glycopolymers for Efficient Inhibition of Galectin-3: In Vitro Proof of Efficacy Using Suppression of T Lymphocyte Apoptosis and Tumor Cell Migration. Biomacromolecules, 21 (8), 3122-3133 (2020). IF 6,092

Silybin/silymarin & other natural products

  • Simona Dobiasová, Kateřina Řehořová, Denisa Kučerová, David Biedermann, Kristýna Káňová, Lucie Petrásková, Kamila Koucká, Radka Václavíková, Kateřina Valentová, Tomáš Ruml, Tomáš Macek, Vladimír Křen, Jitka Viktorová: Multidrug Resistance Modulation Activity of Silybin Derivatives and Their Anti-inflammatory Potential. Antioxidants, 9 (5), 455:1-25 (2020).
  • Antonia Diukendjieva, Maya M. Zaharieva, Mattia Mori, Petko Alov, Ivanka Tsakovska, Tania Pencheva, Hristo Najdenski, Vladimír Křen, Chiara Felici, Francesca Bufalieri, Lucia Di Marcotullio, Bruno Botta, Maurizio Botta, Ilza Pajeva: Dual SMO/BRAF Inhibition by Flavonolignans from Silybum marianum. Antioxidants, 9 (5), 384:1-12 (2020).
  • Šuk Jakub, Jašprová Jana, Biedermann David, Petrásková Lucie, Valentová Kateřina, Křen Vladimír, Muchová Lucie, Vítek Libor: Isolated Silymarin Flavonoids Increase Systemic and Hepatic Bilirubin Concentrations and Lower Lipoperoxidation in Mice. Oxidative Medicine and Cellular Longevity, Volume 2019, Article ID 6026902, 1-12 (2019).
  • Fenclová Marie, Nováková Alena, Viktorová Jitka, Jonatová Petra, Dzuman Zbyněk, Ruml Tomáš, Křen Vladimír, Hajšlová Jana, Vítek Libor, Stránská-Zachariášová Milena: Poor chemical and microbiological quality of the commercial milk thistle-based dietary supplements may account for their reported unsatisfactory and non-reproducible clinical outcomes. Scientific Reports, 9, 11118:1-12 (2019).
  • Viktorová Jitka, Stránská-Zachariášová Milena, Fenclová Marie, Vítek Libor, Hajšlová Jana, Křen Vladimír, Ruml Tomáš: Complex Evaluation of Antioxidant Capacity of Milk Thistle Dietary Supplements. Antioxidants, 8 (8), 317:1-18 (2019).
  • Biedermann David, Moravcová Veronika, Valentová Kateřina, Kuzma Marek, Petrásková Lucie, Císařová Ivana, Křen Vladimír: Oxidation of flavonolignan silydianin to unexpected lactone-acid derivative. Phytochemistry Letters, 30, 14-20 (2019).
  • Mazzaferro S. Laura, Weiz Gisela, Braun Lucas, Kotik Michael, Pelantová Helena, Křen Vladimír, Breccia D. Javier: Enzyme-mediated transglycosylation of rutinose (6-O-α-L-rhamnosyl-D-glucose) to phenolic compounds by a diglycosidase from Acremonium sp. DSM 24697. Biotechnology and Applied Biochemistry, 66 (1), 53-59 (2019).
  • Chambers S. Christopher, Biedermann David, Valentová Kateřina, Petrásková Lucie, Viktorová Jitka, Kuzma Marek, Křen Vladimír: Preparation of Retinoyl-Flavonolignan Hybrids and Their Antioxidant Properties. Antioxidants, 8 (7), 236:1-12 (2019).
  • Heřmánková Eva, Zatloukalová Martina, Biler Michal, Sokolová Romana, Bancířová Martina, Tzakos G. Andreas, Křen Vladimír, Kuzma Marek, Trouillas Patrick, Vacek Jan: Redox properties of individual quercetin moieties. Free Radical Biology and Medicine, 143, 240-251 (2019).
  • Diukendjieva Antonia, Alov Petko, Tsakovska Ivanka, Pencheva Tania, Richarz Andrea, Křen Vladimír, Cronin T.D. Mark, Pajeva Ilza: In vitro and in silico studies of the membrane permeability of natural flavonoids from Silybum marianum (L.) Gaertn. and their derivatives. 
  • J. Vrba, B. Papouskova, L. Roubalova, M. Zatloukalova, D. Biedermann, V. Kren, K. Valentova, J. Ulrichova, J. Vacek: Metabolism of flavonolignans in human hepatocytes. J. Pharm. Biomed. Anal. 152, 94–101 (2018).
  • M. C. Catapano, V. Tvrdý, J. Karlíčková, T. Migkos, K. Valentová, V. Křen, P. Mladěnka: The stoichiometry of isoquercitrin complex with iron or copper is highly dependent on experimental conditions. Nutrients 9, No. 1193 (2017).
  • M. Biler, D. Biedermann, K. Valentová, V. Křen, M. Kubala: Quercetin and its analogues: Optical and acido-basic properties. Physical Chemistry Chemical Physics 19, 26870–26879 (2017).
  • C. S. Chambers, V. Holečková, L. Petrásková, D. Biedermann, K. Valentová, M. Buchta, V. Křen: The silymarin composition… and why does it matter??? Food Res. Int. 100, 339–353 (2017).
  • R. Sokolová, J. Kocábová, P. Marhol, J. Fiedler, D. Biedermann, J. Vacek, V. Křen: Oxidation of natural bioactive flavonolignan 2,3-dehydrosilybin. An electrochemical mechanistic study. The Journal of Physical Chemistry, B 121, 6841–6846 (2017).
  • Egea J, Křen V, , Valentová K, Daiber A. et al.: European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS). Redox Biology 13, 94–162 (2017).
  • E. Heřmánková-Vavříková, A. Křenková, L. Petrásková, C. S. Chambers, J. Zápal, M. Kuzma, K. Valentová, V. Křen: Synthesis and antiradical activity of isoquercitrin esters with aromatic acids and their homologues. Int. J. Mol. Sci. 18, 1074 (2017).
  • L. Roubalová, A. Dinkova-Kostova, D. Biedermann, V. Kren, J. Ulrichová, J. Vrba: Flavonolignan 2,3-dehydrosilydianin activates Nrf2 and upregulates NAD(P)H:quinone oxidoreductase 1 in Hepa1c1c7 cells. Phytoterapia 119, 115–120 (2017).
  • K. Filippopoulou, N. Papaevgeniou, M. Lefaki, A. Paraskevopoulou, D. Biedermann, V. Kren, N. Chondrogianni: 2,3-Dehydrosilybin A and B as a pro-longevity and anti-aggregation compound. Free Radical Biology & Medicine 103, 256–267 (2017).
  • E. Vavříková, V. Křen, L. Jezova-Kalachova, M. Biler, B. Chantemargue, M. Pyszková, S. Riva, M. Kuzma, K. Valentová, J. Ulrichová, J. Vrba, P. Trouillas, J. Vacek: Novel flavonolignan hybrid antioxidants: From enzymatic preparation to molecular rationalization. Eur. J. Med. Chem. 127, 263–274 (2017).
  • D. Biedermann, M. Buchta, V. Holeckova, D. Sedlak, K. Valentova, J. Cvacka, L. Bednarova, A. Krenkova, M. Kuzma, C. Skuta, Z. Peikerova, P. Bartunek, V. Kren: Silychristin: Skeletal alterations and biological activities. J. Nat. Prod. 79, 3086–3092 (2016).
  • L. Roubalová; D. Biedermann; B. Papoušková; J. Vacek; M. Kuzma; V. Křen; J. Ulrichová; A. T DinkovaKostova; J. Vrba: A semisynthetic flavonoid 7-O-galloylquercetin activates Nrf2 and induces Nrf2-dependent gene expression in RAW264.7 and Hepa1c1c7 cells. Chemico-Biological Interactions 260, 58–66 (2016).
  • N. Cibiček, L. Roubalová, J. Vrba, M. Zatloukalová, J. Ehrmann, J. Zapletalová, R. Večeřa, V. Křen, J. Ulrichová: Protective effect of isoquercitrin against acute dextran sulfate sodium-induced rat colitis depends on the severity of tissue damage. Pharmacological Reports 68, 1197–1204 (2016).
  • M. Zatloukalová, E. Vavříková, A. D. Rodrigues Pontinha, J. Coufal, V. Křen, M. Fojta, J. Ulrichová, A. M. Oliveira-Brett, J. Vacek: Flavonolignan conjugates as DNA-binding ligands and topoisomerase I inhibitors: Electrochemical and electrophoretic approaches. Electroanalysis 28, 2866–2874 (2016).
  • E. Vavříková, F. Langschwager, L. Jezova-Kalachova, A. Křenková, B. Mikulová, M. Kuzma, V. Křen, K. Valentová: Isoquercitrin esters with mono- or dicarboxylic acids: enzymatic preparation and properties. Int. J. Mol. Sci. 17, 899 (2016).
  • M. Biler, P. Trouillas, D. Biedermann, V. Křen, M. Kubala: Tunable optical properties of silymarin flavonolignans. J. Photochem. Photobiol. A: Chemistry. 328, 154–162 (2016).
  • R. Sokolová, J. Tarábek, B. Papoušková, J. Kocábová, J. Fiedler, J. Vacek, P. Marhol, E. Vavříková, V. Křen: Electrochemical oxidation of the flavonolignan silybin. In situ EPR evidence of the spin-trapped silybin radical. Electrochimica Acta 205, 118–123 (2016).
  • G. Huang, S. Schramm, J. Heilmann, D. Biedermann, V. Křen, M. Decker: Unconventional application of the Mitsunobu reaction: Selective flavonolignan dehydration yielding hydnocarpins. Beilstein J. Org. Chem. 12, 662–669 (2016). doi:10.3762/bjoc­.12.66
  • D. Karas, R. Gažák, K. Valentová, C. S. Chambers, V. Pivodová, D. Biedermann, A. Křenková, I. Oborná, M. Kuzma, J. Cvačka, J. Ulrichová, V. Křen: Effects of 2,3-dehydrosilybin and its galloyl ester and methyl ether derivatives on human umbilical vein endothelial cells. J. Nat. Prod. 79, 812–820 (2016).
  • K. Valentová, P. Šíma, Z. Pavlíčková, J. Křižan, K. Malachová, J. Červeň, V. Křen: (Anti)Mutagenic Properties and Modulation of Murine Immune System by Quercetin, Isoquercitrin and Rutin. Journal of the Science of Food and Agriculture (J. Sci. Food Agric). 96, 1492–1499 (2016)
  • M. Pyszkova; M. Biler; D. Biedermann; K. Valentova; M. Kuzma; J. Ulrichova; R. Sokolova; M. Mojovic; A. Popovic-Bijelic; M. Kubala; P. Trouillas; V. Kren; J. Vacek: Flavonolignan 2,3-Dehydroderivatives: Preparation, Antiradical and Cytoprotective Activity. Free Radical Biology and Medicine 90(1), 114–125 (2016).
  • L. Roubalová, K. Purchartová, B. Papoušková, J. Vacek, V. Křen, J. Ulrichová, J. Vrba: Sulfation modulates the cell uptake, antiradical activity and biological effects of flavonoids in vitro: An examination of quercetin, isoquercitrin and taxifolin. Bioorg. Med. Chem. 23, 5402–5409 (2015).
  • Cynthia Tilley, Gagan Deep, Chapla Agarwal, Michael F. Wempe, David Biedermann, Kateřina Valentová, Vladimir Kren, Rajesh Agarwal: Silibinin and its 2,3-Dehydro-derivative Inhibit Basal Cell Carcinoma Growth via Suppression of Mitogenic Signaling and Transcription Factors Activation. Molecular Carcinogenesis 55, 3–14 (2016)
  • C. S. Chambers, K. Valentová, V. Křen: Non-Taxifolin Derived Flavonolignans: Phytochemistry and Biology. Curr. Pharm. Design 21(38), 5489–5500 (2015).
  • P. Marhol, P. Bednář, P. Kolářová, R. Večeřa, J. Ulrichová, E. Tesařová, E. Vavříková, M. Kuzma, V. Křen: Pharmacokinetics of pure silybin diastereoisomers and identification of their metabolites in rat plasma. Journal of Functional Foods 14, 570–580 (2015).
  • E. Gabrielova, V. Kren, M. Jaburek, M. Modriansky: Silymarin component 2,3-dehydrosilybin attenuates cardiomyocyte damage following hypoxia/reoxy­genation by limiting oxidative stress. Physiol. Res. 64, 79–91 (2015).
  • D. Biedermann, E. Vavříková, L. Cvak, V. Křen: Chemistry of silybin. Nat. Prod. Rep. 31(9), 1138–1156 (2014).
  • K. Valentová, J. Vrba, M. Bancířová, J. Ulrichová, V. Křen: Isoquercitrin: Pharmacology, toxicology, and metabolism. Food Chem. Toxicol. 68, 267–282 (2014).
  • M. Novotná, R. Gažák, D. Biedermann, F. Di Meo, P. Marhol, M. Kuzma, L. Bednárová, K. Fuksová, P. Trouillas, V. Křen: Cis-trans isomerization of silybins A and B. Beilstein J. Org. Chem. 10, 1047–1063 (2014).
  • E. Vavříková, J. Vacek, K. Valentová, P. Marhol, J. Ulrichová, M. Kuzma, V. Křen: Chemo-enzymatic synthesis of silybin and 2,3-dehydrosilybin dimers. Molecules 19(4), 4115–4134 (2014).
  • Křenek K., Marhol P., Peikerová Ž., Křen V., Biedermann D. Preparatory separation of the silymarin flavonolignans by Sephadex LH-20. Food. Res. Internat. 65, 115–120 (2014).
  • C. Charrier, R. Azerad, P. Marhol, K. Purchartová, M. Kuzma, V. Křen: Microbial preparation of silybin Phase II metabolites: Glucuronides. J. Mol. Cat.: B-Enzymat. 102, 167–173 (2014).
  • J. Vacek, M. Zatloukalova, T. Desmier, V. Nezhodova, J. Hrbac, M. Kubala, V. Kren, J. Ulrichova, P. Trouillas: Antioxidant, Metal-Binding and DNA-damaging Properties of Flavonolignans: A Joint Experimental and Computational Highlight based on 7-O-Galloylsilybin. Chem.-Biol. Interact. 205, 173–180 (2013).
  • V. Křen, P. Marhol, K. Purchartová, E. Gabrielová, M. Modrianský: Biotransformation of silybin and its congeners. Current Drug. Metab. 14(10), 1009–1021 (2013).
  • M. Zatloukalová, T. A. Enache, V. Křen, J. Ulrichová, J. Vacek, A. M. Oliveira-Brett: Effect of 3-O-galloyl substitution on the electrochemical oxidation of quercetin and silybin galloyl esters at glassy carbon electrode. Electroanalysis 25, 1621–1628 (2013).
  • P. Marhol, P. Bednář, E. Tománková, B. Papoušková, J. Stávek, P. Híc, P. Barták, V. Křen, J. Balík: Preparative purification and isolation of pyranoanthocyanins from red wine and evaluation of their antioxidant activity. Chromatographia 76, 1107–1115, (2013).
  • J. Vacek, B. Papoušková, J. Vrba, M. Zatloukalová, V. Křen, J. Ulrichová: LC-MS Metabolic Study on Quercetin and Taxifolin Galloyl Esters Using Human Hepatocytes as Toxicity and Biotransformation In vitro Cell Model. Journal of Pharmaceutical and Biomedical Analysis 86, 135–142 (2013).
  • Petr Marhol, Aloysius F. Hartog, Michael A. van der Horst, Ron Wever, Kateřina Purchartová, Kateřina Fuksová, Marek Kuzma, Josef Cvačka, Vladimír Křen Preparation of silybin and isosilybin sulfates by sulfotransferase from Desulfitobacterium hafniense. J. Mol. Catal., B: Enzymat 89, 24–27 (2013).
  • Vrba, J.; Gažák, R.; Kuzma, M.; Papoušková, B.; Vacek, J.; Weiszenstein, M.; Křen, V.; Ulrichová, J. A novel semisynthetic flavonoid 7-O-galloyltaxifolin upregulates heme oxygenase-1 in RAW264.7 cells via MAPK/Nrf2 pathway. J. Med. Chem. 56(3), 856–866 (2013).
  • K. Purchartová, L. Engels, P. Marhol, K. Slámová, M. Šulc, M. Kuzma, L. Elling, V. Křen: Enzymatic preparation of silybin phase II metabolites: Sulfation using aryl sulfotransferase from rat liver. Appl. Microbiol. Biotechnol. 97, 10391–10398 (2013).
  • C. Agarwal, R. Wadhwa, G. Deep, D. Biedermann, R. Gažák, V. Křen, R. Agarwal: Anti-cancer efficacy of silybin derivatives – A structure-activity relationship. PlosOne 8(3), e60074 (2013).
  • R. Gažák, P. Trouillas, D. Biedermann, K. Fuksová, P. Marhol, M. Kuzma, V. Křen: Base-catalyzed oxidation of silybin and isosilybin into 2,3-dehydro-derivatives. Tetrahedron Lett. 54 (4), 315–317 (2013).
  • R. Gažák, K. Fuksová, P. Marhol, M. Kuzma, R. Agarwal, V. Křen: Preparative method for isosilybin isolation based on enzymatic kinetic resolution of silymarin mixture. Proc. Biochem. 48, 184–189 (2013).
  • Eleni Theodosiou, Kateřina Purchartová, Haralambos Stamatis, Fragiskos Kolisis, Vladimír Křen Bioavailability of silymarin flavonolignans: Drug formulations and biotransformation Phytochemistry Rev. 13, 1–18 (2014).
  • J. Vacek, B. Papoušková, P. Kosina, J. Vrba, V. Křen, J. Ulrichová: Biotransformation of flavonols and taxifolin in hepatocyte in vitro systems as determined by liquid chromatography with various stationary phases and electrospray ionization-quadrupole time-of-flight mass spectrometry. J. Chromatogr. B 899, 109–115 (2012).
  • K. Slámová, P. Bojarová, D. Gerstorferová, B. Fliedrová, J. Hofmeisterová, M. Fiala, P. Pompach, V. Křen: Sequencing, cloning and high-yield expression of a fungal β-N-acetylhexosami­nidase in Pichia pastoris. Protein Expression and Purification 82, 212–217 (2012).
  • J. Vrba, V. Kren, J. Vacek, B. Papouskova, J. Ulrichova: Quercetin, quercetin glycosides and taxifolin differ in their ability to induce AhR activation and CYP1A1 expression in HepG2 cells. Phytotherapy Res. 26(11), 1746–1752 (2012).
  • R. Gažák, K. Valentová, K. Fuksová, P. Marhol, M. Kuzma, M. A. Medina, I. Oborná, J. Ulrichová, V. Křen: Synthesis and Antiangiogenic Activity of New Silybin Galloyl Esters. J. Med. Chem. 54, 7397–7407 (2011).
  • M. Zatloukalová, V. Kren, R. Gazak, M. Kubala, P. Trouillas, J. Ulrichova; J. Vacek: Electrochemical investigation of flavonolignans and study of their interactions with DNA in the presence of Cu(II). Bioelectrochemistry 82, 117–124 (2011).
  • P. Jančová, E. Anzenbacherová, M. Šiller, V. Křen, P. Anzenbacher, V. Šimánek: Stereoselective metabolism of silybin by UDP glucuronosyltran­sferases in vitro. Xenobiotica 41(9), 743–751 (2011).
  • E. Gabrielová, M. Jabůrek, R. Gažák, J. Vostálová, J. Ježek, V. Křen, M. Modrianský: Dehydrosilybin attenuates the production of ROS in rat cardiomyocyte mitochondria with an uncoupler-like mechanism. J. Bioenerg. Biomembr. 42, 499–509 (2010).
  • R. Gažák, P. Marhol, K. Purchartová, D. Monti, D. Biedermann, S. Riva, L. Cvak, V. Křen: Large-scale separation of silybin diastereoisomers using lipases. Proc. Biochem. 45, 1657–1663 (2010).
  • D. Monti, R. Gažák, P. Marhol, D. Biedermann, K. Purchartová, M. Fedrigo, S. Riva, V. Křen, Enzymatic Kinetic Resolution of Silybin Diastereoisomers. J. Nat. Prod. 73(4), 613–619 (2010).
  • R. Gažák, K. Purchartová, P. Marhol, L. Živná, P. Sedmera, K. Valentová, N. Kato, H. Matsumura, K. Kaihatsu, V. Křen: Antioxidant and antiviral activities of silybin fatty acid conjugates. Eur. J. Med. Chem. 45, 1059–1067 (2010).
  • V. Křen, R. Gažák, K. Purchartová, P. Marhol, D. Biedermann, P. Sedmera Chemoenzymatic preparative separation of silybin A and B. J. Mol. Catal. B: Enzymat. 61, 247–251 (2009).
  • Eleni Theodosiou, Maria H. Katsoura, Heleni Loutrari, Kateřina Purchartová, Vladimír Křen, Fragiskos N. Kolisis, Haralambos Stamatis: Enzymatic preparation of acylated derivatives of silybin in organic and ionic liquids media and evaluation of their anti-tumor proliferative activity. Biocat. Biotrans. 27 (3), 161–169, (2009).
  • R. Gažák, P. Sedmera, M. Vrbacký, J. Vostálová, Z. Drahota, P. Marhol, D. Walterová, V. Křen: Molecular mechanisms of silybin and 2,3-dehydrosilybin antiradical activity – role of individual hydroxyl groups. Free Rad. Biol. Med. 46, 745–758 (2009).
  • Patrick Trouillas, Philippe Marsal, Alena Svobodová, Jitka Vostálová, Jan Hrbáč, Radek Gažák, Vladimír Křen, Roberto Lazzaroni, Jean-Luc Duroux, Petr Sedmera, Daniela Walterová: Mechanism of The Antioxidant Action of Silybin and 2,3-Dehydrosilybin Flavonolignans: A Joint Experimental and Theoretical Study. Journal of Physical Chemistry A. 112, 1054–1063 (2008).
  • R. Gažák, P. Sedmera, M. Marzorati, S. Riva, V. Křen: Laccase-mediated dimerization of the flavonolignan silybin. J. Mol. Catal./B Enzymatic 50, 87–92 (2008).
  • Kateřina Valentová, David Stejskal, Josef Bartek, Svatava Dvořáčková, Vladimír Křen, Jitka Ulrichová, Vilím Šimánek: Maca (Lepidium meyenii) and yacon (Smallanthus sonchifolius) in combination with silymarin as food supplements: in vivo safety assessment. Food and Chemical Toxicology 46, 1006–1013 (2008).
  • R. Gažák, D. Walterová, V. Křen: Silybin and silymarin – new and emerging applications in medicine. Curr. Med. Chem. 14 (3), 315–338 (2007).
  • P. Simerská, D. Monti, I. Čechová, H. Pelantová, M. Macková, K. Bezouška, S. Riva, V. Křen: Induction and characterization of an unusual -D-galactosidase from Talaromyces flavus. J. Biotechnol. 128 (1), 61–71 (2007).
  • P. Džubák, M. Hajdúch, R. Gažák, A. Svobodová, J. Psotová, D. Walterová, P. Sedmera, V. Křen: New derivatives of silybin and 2,3-dehydrosilybin and their cytotoxic and P-glycoprotein modulatory activity. Bioorg. Med. Chem. 14 (11) 3793–3810 (2006).
  • K. Valentová, D. Buckiová, V. Křen, J. Pěknicová, J. Ulrichová, V. Šimánek: The in vitro biological activity of Lepidium meyenii extracts. Cell Biol. Toxicol. 22, 91–99 (2006).

Biotransformations

  • Chambers S. Christopher, Viktorová Jitka, Řehořová Kateřina, Biedermann David, Turková Lucie, Macek Tomáš, Křen Vladimír, Valentová Kateřina: Defying Multidrug Resistance! Modulation of related Transporters by Flavonoids and Flavonolignans. Journal of Agricultural and Food Chemistry, 68 (7), 1763-1779 (2020).
  • Kapešová Jana, Petrásková Lucie, Markošová Kristína, Rebroš Martin, Kotik Michael, Bojarová Pavla, Křen Vladimír: Bioproduction of Quercetin and Rutinose Catalyzed by Rutinosidase: Novel Concept of „Solid State Biocatalysis“. International Journal of Molecular Sciences, 20 (5), 1112 (1-15) (2019).
  • K. Valentová, K. Káňová, F. Di Meo, H. Pelantová, C. S. Chambers, L. Rydlová, L. Petrásková, A. Křenková, J. Cvačka, P. Trouillas, V. Křen: Chemoenzymatic preparation and biophysical properties of sulfated quercetin metabolites. Int. J. Mol. Sci. 18, No. 2231
  • I. Bassanini, J. Krejzová, W. Panzeri, V. Křen, D. Monti, S. Riva: A sustainable one-pot two-enzyme synthesis of naturally occurring arylalkyl glucosides. ChemSusChem 10, 2040–2045 (2017).
  • I. Bassanini, P. Gavezzotti, D. Monti, J. Krejzová, V. Kren, S. Riva: Laccase-catalyzed dimerization of glycosylated lignols. J. Mol. Catal. B: Enzymat. 134, 295–301 (2016).
  • E. Vavříková, P. Gavezzotti, K. Purchartová, K. Fuksová, D. Biedermann, M. Kuzma, S. Riva, V. Křen: Regioselective alcoholysis of silychristin acetates catalyzed by lipases. Int. J. Mol. Sci. 16, 11983–11995 (2015).
  • K. Purchartová, K. Valentová, H. Pelantová, P. Marhol, J. Cvačka, L. Havlíček, A. Křenková, E. Vavříková, D. Biedermann, C. S. Chambers, V. Křen: Prokaryotic and Eukaryotic Aryl Sulfotransferases: Sulfation of Quercetin and its Derivatives. ChemCatChem 7, 3152–3162 (2015).
  • K. De Winter, L. Van Renterghem, K. Wuyts, H. Pelantová, V. Křen, W. Soetaert, T. Desmet: Chemoenzymatic synthesis of β-D-glucosides using cellobiose phosphorylase from Clostridium thermocellum. Adv. Synth. Catal. 357 (8), 1961–1969 (2015).
  • P. Gavezzotti, F. Bertacchi, G. Fronza, V. Křen, D. Monti, S. Riva: Laccase-catalyzed dimerization of piceid, a resveratrol glucoside, and its further enzymatic elaboration. Adv. Synth. Catal. 357 (8), 1831–1839 (2015).
  • P. Gavezzotti, E. Vavříková, K. Valentová, G. Fronza, T. Kudanga, M. Kuzma, S. Riva, D. Biedermann, V. Křen Enzymatic oxidative dimerization of silymarin flavonolignans. J. Mol. Catal. B: Enzymat. 109, 24–30 (2014).
  • K. De Winter, T. Desmet, T. Devlamynck, L. Van Renterghem, T. Verhaeghe, H. Pelantová, V. Křen, W. Soetaert: Biphasic catalysis with disaccharide phosphorylases: chemoenzymatic synthesis of α-D-glucosides using sucrose phosphorylase. Organic Process Research & Development 18(6), 781–787 (2014).
  • M. Rebroš, A. Pilníková, D. Šimčíková, L. Weignerová, R. Stloukal, V. Křen, M. Rosenberg: Recombinant α-L-rhamnosidase of Aspergillus terreus immobilization in polyvinylalcohol hydrogel and its application in rutin derhamnosylation. Biocat. Biotrans 31(6), 329–334 (2013).
  • M. Kotik, A. Archelas, V. Faměrová, P. Oubrechtová, V. Křen: Laboratory evolution of an epoxide hydrolase – Towards an enantioconvergent biocatalyst. J. Biotechnol. 156, 1–10 (2011).
  • B. Uhnáková, R. Ludwig, J. Pěknicová, L. Homolka, L. Lisá, M. Šulc, A. Petříčková, F. Elzeinová, H. Pelantová, D. Monti, V. Křen, D. Haltrich, L. Martínková: Biodegradation of tetrabromobisphenol A by oxidases in basidiomycetous fungi and estrogenic activity of the biotransformation products. Bioresource Technol. 102, 9409–9415 (2011).
  • P. Marhol, R. Gažák, P. Bednář, V. Křen: Narrow-bore core-shell particles and monolithic columns in the analysis of silybin diastereoisomers. J. Sep. Sci. 34, 2206–2213 (2011).
  • K. Purchartová, P. Marhol, R. Gažák, D. Monti, S. Riva, M. Kuzma, V. Křen Regioselective alcoholysis of silybin A and B acetates with lipases. J. Mol. Catal. B: Enzymat. 71, 119–123 (2011).
  • P. Košinová, R. Gažák, J.-L. Duroux, R. Lazzaroni, V. Křen, X. Assfeld, P. Trouillas Dimerisation Process of Silybin-Type Flavonolignans: A Theoretical Highlighting. ChemPhysChem 12, 1135–1142 (2011).
  • Bossu I., Šulc M., Křenek K., Dufour E., Garcia J., Berthet N., Dumy P., Křen V., Renaudet O.: Dendri-RAFTs: a second generation of cyclopeptide-based glycoclusters. Org. Biomol. Chem. 9, 1948–1959 (2011).
  • R. Myjavcová, P. Marhol, V. Křen, V. Šimánek, J. Ulrichová, I. Palíková, B. Papoušková, K. Lemr, Petr Bednář: Analysis of anthocyanin pigments in Lonicera (Caerulea) extracts using chromatographic fractionation followed by microcolumn liquid chromatography – hybrid quadrupole time-of flight mass spectrometer -mass spectrometry. J. Chrom. A 1217, 7932–7941 (2010).
  • V. Křen: Silibinin chirality. J. Photochem. Photobiol. A: Chemistry 203, 222–223 (2009).
  • L. Martínková, V. Křen: Biotransformations with nitrilases. Curr. Opin. Chem. Biol. 14, 130–137 (2010).
  • L. Martínková, B. Uhnáková, M. Pátek, J. Nešvera, V. Křen: Biodegradation potential of the genus Rhodococcus. Environment International 35, 162–177 (2009).
  • Palíková I., Heinrich J., Bednár P., Marhol P., Kren V., Cvak L., Valentová K., Ruzicka Filip, Holá V., Kolár M., Simánek V., Ulrichová J. Constituents and Antimicrobial Properties of Blue Honeysuckle, a Novel Source for Phenolic Antioxidants. J. Agric. Food Chem. 56 (24), 11883–11889 (2008).
  • L. Martínková, V. Vejvoda, V. Křen: Selection and screening for enzymes of nitrile metabolism. J. Biotechnol. 133, 318–326 (2008).
  • D. Kubáč, O. Kaplan, V. Elišáková, M. Pátek, V. Vejvoda, K. Slámová, A. Tóthová, M. Lemaire, E. Gallienne, S. Lutz-Wahl, L. Fischer, M. Kuzma, H. Pelantová, S. van Pelt, J. Bolte, V. Křen, L. Martínková: Biotransformation of nitriles to amides using soluble and immobilized nitrile hydratase from Rhodococcus erythropolis A4. J. Mol. Catal./B Enzymatic. 50, 107–113 (2008).
  • V. Vejvoda, O. Šveda, O. Kaplan, V. Přikrylová, V. Elišáková, M. Himl, H. Pelantová, M. Kuzma, V. Křen, L. Martínková: Biotransformation of heterocyclic dinitriles by Rhodococcus erythropolis and fungal nitriles. Biotechnol. Lett. 29, 1119–1124 (2007).
  • R. Gažák, V. Křen, P. Sedmera, D. Passarella, M. Novotná, B. Danieli: Studies on oxidation of ergot alkaloids. Oxidation and desaturation of dihydrolysergol – stereochemical requirements. Tetrahedron 63, 10466–10478 (2007).
  • V. Vejvoda, O. Kaplan, D. Kubáč, V. Křen, L. Martínková: Immobilization of fungal nitrilase and bacterial amidase – two enzymes working in accord. Biocatalysis and Biotransformation 24(6), 414–418 (2006).
  • O. Kaplan, V. Vejvoda, O. Plíhal, P. Pompach, D. Kavan, P. Fialová, K. Bezouška, M. Macková, M. Cantarella, V. Jirků, V. Křen, L. Martínková: Purification and characterization of a nitrilase from Aspergillus niger K10. Appl. Microbiol. Biotechnol. 73 (3), 567–575 (2006).

Glycosciences

  • Vladimír Křen, Pavla Bojarová: Editorial: Biocatalysis: „A Jack of all Trades…“. International Journal of Molecular Sciences, 21 (14), 5115: 1-3 (2020).
  • Katerina Brodsky, Michal Kutý, Helena Pelantová, Josef Cvačka, Martin Rebroš, Michael Kotík, Ivana Kutá Smatanová, Vladimír Křen, Pavla Bojarová: Dual Substrate Specificity of the Rutinosidase from Aspergillus niger and the Role of Its Substrate Tunnel. International Journal of Molecular Sciences, 21 (16), 5671: 1-21 (2020).
  • Tavares Marina Rodrigues, Bláhová Markéta, Sedláková Lieselotte, Elling Lothar, Pelantová Helena, Konefal Rafal, Etrych Tomáš, Křen Vladimír, Bojarová Pavla, Chytil Petr: High-Affinity N-(2-Hydroxylpropyl)methacrylamide Copolymers with Tailored N-Acetyllactosamine Presentation Discriminate between Galectins. Biomacromolecules, 21 (2), 641-652 (2020).
  • Bassanini Ivan, Kapešová Jana, Petrásková Lucie, Pelantová Helena, Markošová Kristína, Rebroš Martin, Valentová Kateřina, Kotik Michael, Káňová Kristýna, Bojarová Pavla, Cvačka Josef, Turková Lucie, Ferrandi E. Erica, Bayout Ikram, Riva Sergio, Křen Vladimír: Glycosidase-Catalyzed Synthesis of Glycosyl Esters and Phenolic Glycosides of Aromatic Acids. Advanced Synthesis and Catalysis, 361 (11), 2627-2637 (2019).
  • Garcia-Oliva Cecilia, Hoyos Pilar, Petrásková Lucie, Kulik Natalia, Pelantová Helena, Cabanillas H. Alfredo, Rumbero Ángel, Křen Vladimír, Hernáiz J. María, Bojarová Pavla: Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation. International Journal of Molecular Sciences, 20 (24), 6181: 1-17 (2019).
  • Bojarová Pavla, Kulik Natallia, Slámová Kristýna, Hubálek Martin, Kotik Michael, Cvačka Josef, Pelantová Helena, Křen Vladimír: Selective β-N-acetylhexosaminidase from Aspergillus versicolor—a tool for producing bioactive carbohydrates. Applied Microbiology and Biotechnology, 103 (4), 1737-1753 (2019).
  • Bojarová Pavla, Bruthans Jan, Křen Vladimír: β-N-Acetylhexosaminidases-the wizards of glycosylation. Applied Microbiology and Biotechnology, 103 (19), 7869-7881 (2019).
  • D. Laaf, H. Steffens, H. Pelantová, P. Bojarová, V. Křen, L. Elling: Chemo-enzymatic synthesis of branched N-acetyllactosamine glycan oligomers for galectin-3 inhibition. Adv. Synth. Catal. 359, 4015 – 4024 (2017).
  • D. Laaf, P. Bojarová, H. Pelantová, M. Bienert, C. Schmitz, C. Dey, T. Fischöder, S. Neuss-Stein, W. Jahnen-Dechent, V. Křen, L. Elling: Taylored multivalent neoglycoproteins: Galectin-3 serum diagnostics and angiogenesis inhibitors. Bioconjugate Chemistry 28, 2832–2840 (2017).
  • D. Laaf, P. Bojarová, B. Mikulová, H. Pelantová, V. Křen, L. Elling: Two-step enzymatic synthesis of β-D-N-acetylgalactosamine-(1→4)-D-N-acetylglucosamine (LacdiNAc) chitooligomers for deciphering galectin binding behavior. Adv. Synth. Catal. 359, 2101 – 2108 (2017).
  • P. Bojarová, P. Chytil, B. Mikulová, L. Bumba, R. Konefał, J. Krejzová, K. Slámová, L. Petrásková, L. Kotrchová, H. Pelantová, J. Cvačka, T. Etrych, V. Křen: Glycan-decorated HPMA copolymers as high-affinity lectin ligands. Polymer Chemistry 8, 2647–2658 (2017).
  • P. Bojarová, V. Křen: Sugared biomaterials binding lectins: from in vivo imaging to cancer therapy. Biomaterials Science 4, 1142–1160 (2016).
  • J. Krejzová, N. Kulik, K. Slámová, V. Křen: Expression of human β-N-acetylhexosami­nidase B in yeast eases the search for selective inhibitors. Enzyme Microbial. Technol. 89, 1–6 (2016).
  • B. Lange, A. Šimonová, T. Fischöder, H. Pelantová, V. Křen, L. Elling: Towards keratan sulfate – Chemo-enzymatic cascade synthesis of sulfated N-acetyllactosamine (LacNAc) glycan oligomers. Adv. Synth. Catal. 358 (4), 584–596 (2016)
  • K. De Winter, L. Van Renterghem, K. Wuyts, H. Pelantová, V. Křen, W. Soetaert, T. Desmet: Enzymatic Glycosylation of Phenolic Antioxidants: Phosphorylase-Mediated Synthesis and Characterization. J. Agric. Food Chem. 63, 10131–10139 (2015)
  • B. D. Neher, L. S. Mazzaferro, M. Kotik, J. Oyhenart, P. Halada, V. Křen, J. D. Breccia: Bacteria as source of diglycosidase activity: Actinoplanes missouriensis produces 6-O-α-L-rhamnosyl β-D-glucosidase active on flavonoids. Appl. Microbiol. Biotechnol. 100, 3061–3070 (2016)
  • K. Slámová, J. Krejzová, P. Marhol, L. Kalachova, N. Kulik, H. Pelantová, J. Cvačka, V. Křen: Synthesis of Derivatized Chitooligomers Using Transglycosidases Engineered from the Fungal GH20 β-N-Acetylhexosami­nidase. Adv. Synth. Catal. 357 (8), 1941–1950 (2015).
  • N. Kulik, K. Slámová, R. Ettrich, V. Křen: Computational study of β-N-acetylhexosami­nidase from Talaromyces flavus, a glycosidase with high substrate flexibility. BMC Bioinformatics 16:28 (2015).
  • K. Markošová, L. Weignerova, M. Rosenberg, V. Křen, M. Rebroš: Upscale of recombinant α-L-rhamnosidase production by Pichia pastoris MutS strain. Frontiers in Microbiology 6:1140 (2015).
  • J. Krejzová, L. Kalachová, P. Šimon, H. Pelantová, K. Slámová, V. Křen: Inhibition of microbial β-N-acetylhexosami­nidases by 4-deoxy- and galacto- analogues of NAG-thiazoline. Bioorg. Med. Chem. Lett. 24, 5321–3 (2014)
  • D. Šimčíková, M. Kotik, L. Weignerová, P. Halada, H. Pelantová, K. Adamcová, V. Křen: α-L-Rhamnosyl-β-D-glucosidase (rutinosidase) from Aspergillus niger: Characterization and synthetic potential of a novel diglycosidase. Adv. Synth. Catal. 357, 107–117 (2015).
  • G. Demo, V. Horská, B. Fliedrová, J. Štěpán, J. Koča, L. Weignerová, V. Křen, M. Wimmerová: Protein engineering study of β-mannosidase to set up potential chemically efficent biocatalyst. Glycobiology 24(12), 1301–1311 (2014).
  • K. Křenek, R. Gažák, G. Ch. Daskhan, J. Garcia, M. Fiore, P. Dumy, M. Šulc, V. Křen, O. Renaudet: Access to bifunctionalized biomolecular platforms using oxime ligation. Carbohydr. Res. 393, 9–14 (2014).
  • J. Krejzová, P. Šimon, L. Kalachova, N. Kulik, P. Bojarová, P. Marhol, H. Pelantová, J. Cvačka, R. Ettrich, K. Slámová, V. Křen: Inhibition of GlcNAc-processing glycosidases by C-6-azido NAG-thiazoline and its derivatives. Molecules 19(3), 3471–3488 (2014).
  • D. Rozbeský, J. Krejzová, K. Křenek, J. Prchal, R. Hrabal, M. Kožíšek, L. Weignerová, M. Fiore, P. Dumy, O. Renaudet, V. Křen: Reevaluation of Binding Properties of Recombinant Lymphocyte Receptors NKR-P1A and CD69 to Chemically Synthesized Glycans and Peptides. Int. J. Mol. Sci. 15, 1271–1283 (2014).
  • K. Slámová, N. Kulik, M. Fiala, J. Krejzová-Hofmeisterová, R. Ettrich, V. Křen: Expression, characterization and homology modeling of a novel eukaryotic GH84 β-N-acetylglucosa­minidase from Penicillium chrysogenum. Prot. Express. Purif. 95, 204–210 (2014).
  • A. Šimonová, C. E. Kupper, S. Böcker, A. Müller, K. Hofbauerová, H. Pelantová, L. Elling, V. Křen, P. Bojarová: Chemo-enzymatic synthesis of LacdiNAc dimers of varying length as novel galectin ligands. J. Mol. Cat.: B-Enzymat. 101, 47–55 (2014).
  • K. De Winter, K. Verlinden, V. Křen, L. Weignerová, W. Soetaert, T. Desmet: Ionic liquids as cosolvents for glycosylation by sucrose phosphorylase: balancing acceptor solubility and enzyme stability. Green Chemistry 15, 1949–1955 (2013).
  • Karel De Winter, Daniela Šimčíková, Bram Schalck, Lenka Weignerová, Helena Pelantová, Wim Soetaert, Tom Desmet, Vladimír Křen: Chemoenzymatic synthesis of α-L-rhamnosides using recombinant α-L-rhamnosidase from Aspergillus terreus. Bioresource Technology 147, 640–644 (2013).
  • P. Bojarová, R. R. Rosencrantz, L. Elling, V. Křen: Enzymatic glycosylation of multivalent scaffolds. Chem. Soc. Rev. 42, 4774–4797 (2013).
  • J. Krejzová, P. Šimon, E. Vavříková, K. Slámová, H. Pelantová, S. Riva, V. Spiwok, V. Křen: Synthesis of new C-6-acylated derivatives of NAG-thiazoline and evaluation of their inhibitor activities towards a β-N-acetylhexosami­nidase. J. Mol. Catal., B: Enzymat 87, 128–134 (2013).
  • B. Fliedrová, D. Gerstorferová, V. Křen, L. Weignerová: Production of Aspergillus niger β-mannosidase in Pichia pastoris. Protein Expression and Purification 85, 159–164 (2012).
  • T. Desmet, W. Soetaert, P. Bojarová, V. Kren, L. Dijkhuizen, V. Eastwick-Field, A. Schiller: Enzymatic glycosylation of small molecules: challenging substrates require tailored catalysts. Chemistry – A European Journal 18, 10786–10801 (2012).
  • C. E. Kupper, R. R. Rosencrantz, B. Henßen, H. Pelantová, S. Thönes, A. Drozdová, V. Křen, L. Elling: Chemo-enzymatic synthesis of novel modified poly-N-acetyllactosamine (LacNAc) and N,N-diacetyllactosamine (LacDiNAc) glycan structures by galactose oxidase. Beilstein J. Org. Chem. 8, 712–725 (2012).
  • D. Gerstorferová, B. Fliedrová, P. Halada, P. Marhol, V. Křen, L. Weignerová: Recombinant α-L-rhamnosidase from Aspergillus terreus in selective trimming of rutin. Process Biochem. 47(5), 828–835 (2012).
  • Hoang Anh Nguyen, Thu-Ha Nguyen, V. Křen, V.G.H. Eijsink, D. Haltrich, C.K. Peterbauer: Heterologous expression and characterization of an N-acetyl-β-D-hexosaminidase from Lactococcus lactis ssp. lactis IL1403. Journal of Agricultural and Food Chemistry 60(12), 3275–3281 (2012).
  • K. Křenek, P. Šimon, L. Weignerová, B. Fliedrová, M. Kuzma, V. Křen: Facile synthesis of nitrophenyl 2-acetamido-2-deoxy-α-D-mannopyranosides from ManNAc-oxazoline. Beilstein J. Org. Chem. 8, 428–432 (2012).
  • J. Krejzová, P. Šimon, E. Vavříková, K. Slámová, H. Pelantová, S. Riva, V. Spiwok, V. Křen: Synthesis of new C-6-acylated derivatives of NAG-thiazoline and evaluation of their inhibitor activities towards a β-N-acetylhexosami­nidase. J. Mol. Catal., B: Enzymat 87, 128–134 (2013).
  • J. Krejzová, P. Šimon, E. Vavříková, K. Slámová, H. Pelantová, S. Riva, V. Spiwok, V. Křen: Synthesis of new C-6-acylated derivatives of NAG-thiazoline and evaluation of their inhibitor activities towards a β-N-acetylhexosami­nidase. J. Mol. Catal., B: Enzymat 87, 128–134 (2013).
  • H. Mrazek; O. Benada; P. Man; O. Vanek; V. Kren; K. Bezouska, L. Weignerova: Facile production of Aspergillus niger α-N-acetylgalacto­saminidase in yeast. Protein Expression and Purification 81, 106–114 (2012).
  • P. Bojarová, K. Slámová, K. Křenek, R. Gažák, N. Kulik, R. Ettrich, H. Pelantová, M. Kuzma, S. Riva, D. Adámek, K. Bezouška, V. Křen: Charged hexosaminides as new substrates for
    β-N-acetylhexosami­nidase catalyzed synthesis of immunomodulatory disaccharides. Adv. Synth. Catal. 353, 2409–2420 (2011).
  • C. Rech, R. R. Rosencrantz, K. Křenek, H. Pelantová, P. Bojarová, F.-G. Hanisch, V. Křen, L. Elling: Combinatorial one-pot synthesis of poly-N-acetyllactosamine oligosaccharides with Leloir-glycosyltransfe­rases. Adv. Synth. Catal. 353, 2492–2500 (2011).
  • L. Weignerová, P. Marhol, D. Gerstorferová, V. Křen: Preparatory production of quercetin-3-β-D-glucopyranoside using alkali-tolerant thermostable α-L-rhamnosidase from Aspergillus terreus. Bioresource Technol. 115, 222–227 (2012).
  • P. Bojarová, V. Křen: Glycosidases in carbohydrate synthesis: when organic chemistry falls short. Chimia 65 (1/2), 65–70 (2011).
  • K. Slámová, P. Bojarová, L. Petrásková, V. Křen: β-N-Acetylhexosami­nidase: What‘s in a name…? Biotech. Adv. 28 (6), 682–693 (2010).
  • K. Slámová, R. Gažák, P. Bojarová, N. Kulik, R. Ettrich, H. Pelantová, P. Sedmera, V. Křen: 4-Deoxy-substrates for β-N-acetylhexosami­nidases: How to make use of its loose specificity. Glycobiology 20 (8), 1002–1009 (2010).
  • L. Weignerová, H. Pelantová, D. Manglová, K. Michálková, V. Křen: Condensation reaction catalyzed by α-N-acetylgalacto­saminidase from Aspergillus niger yielding α-N-acetylgalacto saminides. Biocat. Biotrans. 28 (5), 150–155 (2010).
  • V. Křen, P. Bojarová: Glycosyl Azides – Novel Substrates for Enzymatic Transglycosyla­tions. In Practical Methods in Biocatalysis and Biotransformations, (J. Whittall, P. W. Sutton, Eds.); Wiley-VCH: Weinheim, pp. 232–235, 2009.
  • Birgit Sauerzapfe, Karel Křenek Judith Schmiedel, Warren W. Wakarchuk, Helena Pelantová, Vladimir Křen, Lothar Elling: Chemo-enzymatic synthesis of poly-N-Acetyllactosamine (poly-LacNAc) structures and their characterization for galectin-mediated binding of ECM glycoproteins to biomaterial surfaces. Glycoconjugate J. 26, 141–159 (2009).
  • L. Weignerová, P. Simerská, V. Křen: α-Galactosidases and their applications in biotransformations. Biocat. Biotrans. 27 (2), 79–89 (2009).
  • V. Křen, T. Řezanka: Sweet antibiotics – the role of glycosidic residues in antibiotic and antitumor activity and their randomization. FEMS Microbiol. Reviews 32(5), 858–889 (2008).
  • L. Weignerová, T. Filipi, D. Manglová, V. Křen: Induction, purification and characterization of a -N-acetylgalacto­saminidase from Aspergillus niger. Appl. Microbiol. Biotechnol. 79, 769–774 (2008).
  • B. Sauerzapfe, D.-J. Namdjou, T. Schumacher, N. Linden, K. Křenek, V. Křen, L. Elling: Characterization of recombinant fusion constructs of human 1,4-galactosyltran­sferase 1 and the lipase pre-propeptide from Staphylococcus hyicus. J. Mol. Catal./B Enzymatic. 50, 128–140 (2008).
  • P. Bojarová, K. Křenek, M. Kuzma, L. Petrásková, D.-J. Namdjou, L. Elling, V. Křen: N-Acetylhexosamine triad in one molecule: Chemoenzymatic introduction of 2-acetamido-2-deoxy--D-galactopyrano­syluronic acid residue into a complex oligosaccharide. J. Mol. Catal./B Enzymatic. 50, 69–73 (2008).
  • Pavla Bojarová, Lucie Petrásková, Erica Ferrandi, Daniela Monti, Helena Pelantová, Marek Kuzma, Pavla Simerská, and Vladimír Křen: Glycosyl Azides – An Alternative Way to Disaccharides. Adv. Synth. Catal. 349, 1514–1520 (2007).
  • P. Bojarová-Fialová, V. Křen: Enzymatic approaches to O-glycoside introduction: Glycosidases. In Comprehensive Glycoscience (J. P. Kamerling, Ed.); Elsevier: Oxford, 2007, 453–487.
  • L. Petrásková, A. Charvátová, V. Přikrylová, V. Kristová, J. Rauvolfová, L. Martínková, J. Jiménez-Barbero, N. Aboitiz, L. Petruš, V. Křen: Preparative production and separation of 2-acetamido-2-deoxymannopyra­noside-containing saccharides using borate-saturated polyolic exclusion gels. J. Chrom. A 1127, 126 – 136 (2006).
  • M. Petrušová, B. Pribulová, M. Vojtech, M. Matulová, M. Poláková, J. N. BeMiller, V. Křen, L. Petruš: Extension of the Nef reaction to C-glycosylnitro­methanes. Carbohydr. Res. 341, 2019 – 2025 (2006).
  • A. T. Carmona, P. Fialová, V. Křen, R. Ettrich, L. Martínková, A. J. Moreno-Vargas, C. González, I. Robina: Cyanodeoxy-Glycosyl derivatives as substrates for enzymatic reactions. Eur. J. Org. Chem. 2006, 1876–1885 (2006)
  • P. Simerská, M. Kuzma, D. Monti, S. Riva, M. Macková, V. Křen: Unique transglycosylation potential ofextracellular-D-galactosidase from Talaromyces flavus. J. Mol. Catal./B Enzymatic. 39, 128–134 (2006).
  • A. Pišvejcová, C. Rossi, L. Hušáková, V. Křen, S. Riva, D. Monti: -1,4-Galactosyltran­sferase-catalyzed glycosylation of sugar derivatives: new acquisitions J. Mol. Catal: B-Enzymatic. 39, 98–104 (2006).
  • P. Fialová, A. T. Carmona, I. Robina, R. Ettrich, P. Sedmera, V. Přikrylová, L. Hušáková, V. Křen: Glycosyl azides – Novel substrates for enzymatic transglycosyla­tions. Tetrahedron Letters 46, 8715–8718 (2005).
  • P. Bojarová, V. Křen: Glycosidases: a key to tailored carbohydrates. Trends in Biotechnology. 27, 199–209 (2009).
  • K. J. Loft, P. Bojarová, K. Slámová, V. Křen, S. J. Williams: Synthesis of sulfated glucosaminides and use in studying the substrate specificity of sulfatases and fungal -N-acetylhexosami nidases. ChemBioChem 10, 565–576 (2009).
  • L. Weignerová, V. Křen: Enzymatic processing of bioactive glycosides from natural sources. Topics Curr. Chem. 295, 121–146 (2010).
  • V. Křen: Glycoside vs. aglycon “The role of glycosidic residue in biological activity” chapter (56 pp), Fraser-Reid ed. Glycoscience, Springer 2008

 

Last update: July 20, 2021