Doc. RNDr. Pavla Bojarová, PhD.

Senior Scientist, Deputy Head
(+420) 296 442 360
Laboratory of Biotransformation
Institute of Microbiology of the Czech Academy of Sciences
Vídeňská 1083
Praha 4 – Krč
142 20
Czech Republic


  • Charles University Prague, Faculty of Sciences, Dept. of Biochemistry – MSc (2002)
  • Charles University Prague, Faculty of Sciences, Dept. of Biochemistry – PhD (2006)

PhD Thesis

  • Modified Substrates in β-N-Acetylhexosami­nidase-Catalyzed Synthesis
  • PhD. thesis summary (240kB)


Since 2020 Habilitation at Faculty of Biomedical Engineering, Czech Technical University in Prague (habilitation thesis: Novel glyco-nanomaterials for biomedicine and biotechnology)
Since 2019 Lecturer at Faculty of Biomedical Engineering, Czech Technical University in Prague
Since 2010 Lecturer at Department of Biochemistry, Faculty of Science, Charles University in Prague (Biochemistry and Biomedicine of Carbohydrates)
Since 2008 Research fellow in the Laboratory of Biotransformation
2006–2007 Postdoctoral stay at the University of Melbourne, Australia
Since 1999 member of the Laboratory of Biotransformation

Study stays

Year Institute Length
2010 Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen 2 weeks
2006–7 School of Chemistry, Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Australia 13 mths
2006 Directed Enzyme Evolution Course, Inst. Biotechnology, Vilnius 1 week
2005 Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen 7 weeks
2005 Istituto di Chimica del Riconoscimento Molecolare, CNR, Milan 4 weeks
2003 Helmholtz-Institut für Biomedizinische Technik, RWTH Aachen 3 mths
2003 Departamento de Quimica organica, Quimica, Univ. de Sevilla 4 weeks
2002 Institut für Organische Chemie, Universität Hamburg 4 weeks

Prizes & Awards

2019 the prize of the Director of the Institute if Microbiology of the Czech Academy of Sciences, for the best review article
08/2018 invited plenary lecture in the 9th International Congress on Biocatalysis
07/2018 invited lecture in the 29th International Carbohydrate Symposium
2013 the prize of the Director of the Institute of Microbiology of the Czech Academy of Sciences for the best review article
2010, 2012 finalist of the scholarship program L´Oréal Women in Science
2006 the best dissertation thesis in the Institute of Microbiology of the Czech Academy of Sciences

Publications & Presentations

  • 52 papers
  • 4 book chapters
  • 1 patent application
  • 1244 citations (Web of Science, All databases)
  • H-index 21
  • (Co-)author of ca 50 oral or poster presentations in international conferences

Areas of interest

My team is primarily involved in the study of oligosaccharides and glycomimetivs, in their multivalent presentation on various carriers and in the analysis of the properties of the prepared glycoconjugates, especially concerning their interaction with biomedically attractive lectins. At present we are concentrating on galectins and their involvement in tumorigenesis and cardiopathologies. For oligosaccharide synthesis we use chemo-enzymatic synthesis with novel enzymatic tools, which gained favorable synthetic properties or altered substrate specificity through site directed mutagenesis. We study the affinity of glycoconjugates to lectins and lectin-overexpressing cells with a whole series of methods, such as NMR, immunochemistry, biolayer interferometry and flow cytometry.

Selected publications (last 10 years)

  • P. Bojarová a V. Křen. Glycosidases in carbohydrate synthesis: when organic chemistry falls short. Chimia 2011, 65, 65–70.
  • H. Ryšlavá, A. Kalendová, V. Doubnerová, P. Skočdopol, V. Kumar, Z. Kukačka, P. Pompach, O. Vaněk, K. Slámová, P. Bojarová, N. Kulik, R. Ettrich, V. Křen a K. Bezouška. Enzymatic characterization and molecular modeling of an evolutionarily interesting fungal b-N-acetylhexosami­nidase. FEBS J. 2011, 278, 2469–2484.
  • P. Bojarová, K. Slámová, K. Křenek, R. Gažák, N. Kulik, R. Ettrich, H. Pelantová, M. Kuzma, S. Riva, D. Adámek, K. Bezouška a V. Křen. Charged hexosaminides as new substrates for b-N-acetylhexosami­nidase-catalyzed synthesis of immunomodulatory disaccharides. Adv. Synth. Catal. 2011, 353, 2409–2420.
  • C. Rech, R. R. Rosencrantz, K. Křenek, H. Pelantová, P. Bojarová, C. E. Römer, F. G. Hanisch, V. Křen a L. Elling. Combinatorial one-pot synthesis of poly-N-acetyllactosamine oligosaccharides with Leloir-glycosyltransfe­rases. Adv. Synth. Catal. 2011, 353, 2492–2500.
  • A. Drozdová, P. Bojarová, K. Křenek, L. Weignerová, B. Henssen, L. Elling, H. Christensen, H. H. Jensen, H. Pelantová, M. Kuzma, K. Bezouška, M. Krupová, D. Adámek, K. Slámová a V. Křen. Enzymatic synthesis of dimeric glycomimetic ligands of NK cell activation receptors. Carbohydr. Res. 2011, 346, 1599–1609.
  • K. Slámová, P. Bojarová, D. Gerstorferová, B. Fliedrová, J. Hofmeisterová, M. Fiala, P. Pompach a V. Křen. Sequencing, cloning and high-yield expression of a fungal β-N-acetylhexosami­nidase in Pichia pastoris. Prot. Express. Purif. 2012, 82, 212–217.
  • T. Desmet, W. Soetaert, P. Bojarová, V. Křen, L. Dijkhuizen, V. Eastwick-Field a A. Schiller: Enzymatic glycosylation of small molecules. Challenging substrates require tailored catalysts. Chem. Eur. J. 2012, 18, 10786–10801.
  • H. Mrázek, L. Weignerová, P. Bojarová, P. Novák, O. Vaněk a K. Bezouška. Carbohydrate synthesis and biosynthesis technologies for cracking of the glycan code: recent advances. Biotechnol. Adv. 2013, 31, 17–37.
  • P. Bojarová, R. R. Rosencrantz, L. Elling a V. Křen. Enzymatic glycosylation of multivalent scaffolds. Chem. Soc. Rev. 2013, 42, 4774–4797.
  • J. Krejzová, P. Šimon, Ľ. Kalachová, N. Kulik, P. Bojarová, P. Marhol, H. Pelantová, J. Cvačka, R. Ettrich, K. Slámová a V. Křen. Inhibition of GlcNAc-processing glycosidases by C-6-azido-NAG-thiazoline and its derivatives. Molecules 2014, 19, 3471–3488.
  • A. Šimonová, C. E. Kupper, S. Böcker, A. Müller, K. Hofbauerová, H. Pelantová, L. Elling, V. Křen a P. Bojarová. Chemo-enzymatic synthesis of LacdiNAc dimers of varying length as novel galectin ligands. J. Mol. Catal. B: Enzym. 2014, 101, 47–55.
  • P. Bojarová a V. Křen. Sugared biomaterial binding lectins: achievements and perspectives. Biomater. Sci. 2016, 4, 1142–1160.
  • K. Slámová a P. Bojarová. Engineered N-acetylhexosamine-active enzymes in glycoscience. Biochim. Biophys. Acta Gen. Subj. 2017, 1861, 2070–2087.
  • P. Bojarová, P. Chytil, B. Mikulová, L. Bumba, R. Konefał, H. Pelantová, J. Krejzová, K. Slámová, L. Petrásková, L. Kotrchová, J. Cvačka, T. Etrych a V. Křen. Glycan-decorated HPMA copolymers as high-affinity lectin ligands. Polym. Chem. 2017, 8, 2647–2658.
  • D. Laaf, P. Bojarová, B. Mikulová, H. Pelantová, V. Křen a L. Elling. Two-step enzymatic synthesis of b-D-N-acetylgalactosamine-(1→4)-D-N-acetylglucosamine (LacdiNAc) chitooligomers for deciphering galectin binding behavior. Adv. Synth. Catal. 2017, 359, 2101–2108.
  • D. Laaf, P. Bojarová, H. Pelantová, V. Křen a L. Elling. Tailored multivalent neo-glycoproteins: synthesis, evaluation, and application of a library of galectin-3-binding glycan ligands. Bioconjug. Chem. 2017, 28, 2832–2840.
  • D. Laaf, H. Steffens, H. Pelantová, P. Bojarová, V. Křen a L. Elling. Chemo-enzymatic synthesis of branched N-acetyllactosamine glycan oligomers for galectin-3 inhibition. Adv. Synth. Catal. 2017, 359, 4015–4024.
  • L. Bumba, D. Laaf, V. Spiwok, L. Elling, V. Křen a P. Bojarová. Poly-N-acetyllactosamine neo-glycoproteins as nanomolar ligands of human galectin-3: binding kinetics and modeling. Int. J. Mol. Sci. 2018, 19, 372.
  • P. Bojarová, M. R. Tavares, D. Laaf, L. Bumba, L. Petrásková, R. Konefał, M. Bláhová, H. Pelantová, L. Elling, T. Etrych, P. Chytil a V. Křen. Biocompatible glyconanomaterials based on HPMA-copolymer for specific targeting of galectin-3. J. Nanobiotechnol. 2018, 16, 73.
  • D. Laaf, P. Bojarová, L. Elling a V. Křen. Galectin-carbohydrate interactions in biomedicine and biotechnology. Trends Biotechnol. 2019, 37, 402–415.
  • P. Bojarová, N. Kulik, M. Hovorková, K. Slámová, H. Pelantová a V. Křen. The b-N-acetylhexosami­nidase in the synthesis of bioactive glycans: protein and reaction engineering. Molecules 2019, 24, 599.
  • P. Bojarová, N. Kulik, K. Slámová, M. Hubálek, M. Kotik, J. Cvačka, H. Pelantová a V. Křen. Selective β-N-acetylhexosami­nidase from Aspergillus versicolor – a tool for producing bioactive carbohydrates. Appl. Microbiol. Biotechnol. 2019, 103, 1737–1753.
  • J. Kapešová, L. Petrásková, K. Markošová, M. Rebroš, M. Kotik, P. Bojarová a V. Křen. Bioproduction of quercetin and rutinose catalyzed by rutinosidase: novel concept of “solid state biocatalysis”. Int. J. Mol. Sci. 2019, 20, 1112.
  • I. Bassanini, J. Kapešová, L. Petrásková, H. Pelantová, K. Markošová, M. Rebroš, K. Valentová, M. Kotik, K. Káňová, P. Bojarová, J. Cvačka, L. Turková, E. E. Ferrandi, I. Bayout, S. Riva a V. Křen. Glycosidase-catalyzed synthesis of glycosyl esters and phenolic glycosides of aromatic acids. Adv. Synth. Catal. 2019, 361, 2627–2637.
  • P. Bojarová, J. Bruthans a V. Křen. β-N-Acetylhexosami­nidases – the wizards of glycosylation. Appl. Microbiol. Biotechnol. 2019, 103, 7869–7881.
  • Garcia-Oliva, P. Hoyos, L. Petrásková, N. Kulik, H. Pelantová, A. H. Cabanillas, Á. Rumbero, V. Křen, M. J. Hernáiz, P. Bojarová. Acceptor specificity of β-N-acetylhexosaminidase from Talaromyces flavus: a rational explanation. Int. J. Mol. Sci. 2019, 20, 6181.
  • Nekvasilová, I. Andreasová, L. Petrásková, H. Pelantová, V. Křen a P. Bojarová. A novel enzymatic tool for transferring GalNAc moiety onto challenging acceptors. Biochim. Biophys. Acta Prot. Proteom. 2020, 1868, 140319.
  • R. Tavares, M. Bláhová, L. Sedláková, L. Elling, H. Pelantová, R. Konefał, T. Etrych, V. Křen, P. Bojarová a P. Chytil. High-avidity HPMA copolymers with tailored N-acetyllactosamine presentation discriminate between galectins. Biomacromolecules 2020 2020, 21, 641-652.
  • Pachl, J. Kapešová, J. Brynda, L. Biedermannová, H. Pelantová, P. Bojarová, V. Křen, P. Řezáčová a M. Kotik. Rutinosidase from Aspergillus niger: crystal structure and insight into the enzymatic activity. FEBS J. 2020, 287, 3315-3327.
  • Vašíček, V. Spiwok, J. Červený, L. Petrásková, L. Bumba, D. Vrbata, H. Pelantová, V. Křen a P. Bojarová. Regioselective 3-O-substitution of unprotected thiodigalactosides: direct route to galectin Inhibitors. Chem. Eur. J. 2020, 26, 9620-9631.
  • Filipová, P. Bojarová, M. R. Tavares, L. Bumba, L. Elling, P. Chytil, K. Gunár, V. Křen, T. Etrych a O. Janoušková. Glycopolymers for efficient inhibition of galectin-3: in vitro proof of efficacy using suppression of T lmphocyte apoptosis and tumor cell migration. Biomacromolecules 2020, 21, 3122-3133.
  • Křen, P. Bojarová: Biocatalysis: a jack of all trades… Int. J. Mol. Sci. 2020, 21, 5115.
  • Brodsky, M. Kutý, H. Pelantová, J. Cvačka, M. Rebroš, M. Kotik, I. Kutá-Smatanová, V. Křen, a P. Bojarová. Dual substrate specificity of the rutinosidase from Aspergillus niger and the role of its substrate tunnel. Int. J. Mol. Sci. 2020, 21, 5671.
  • Nekvasilová, N. Kulik, N. Rychlá, H. Pelantová, L. Petrásková, Z. Bosáková, J. Cvačka, K. Slámová, V. Křen a P. Bojarová. How site-directed mutagenesis boosted selectivity of a promiscuous enzyme. Adv. Synth. Catal. 2020, 362, 4138-4150.
  • Kapešová, L. Petrásková, N. Kulik, Z. Straková, P. Bojarová, K. Markošová, M. Rebroš, V. Křen a K. Slámová. Transglycosidase activity of glycosynthase-type mutants of a fungal GH20 ß-N-acetylhexosaminidase. Int. J. Biol. Macromol. 2020, 161, 1206-1215.
  • Kotik, K. Brodsky, P. Halada, H. Javůrková, H. Pelantová, D. Konvalinková, P. Bojarová a V. Křen. Access to both anomers of rutinosyl azide using wild-type rutinosidase and its catalytic nucleophile mutant. Catal. Commun. 2021, 149, 106193.
  • Sedlář, M. Trávníčková, R. Matějka, Š. Pražák, Z. Mészáros, Bojarová, L. Bačáková, V. Křen a K. Slámová. Growth factors VEGF-A165 and FGF-2 as multifunctional biomolecules governing cell adhesion and proliferation. Int. J. Mol. Sci. 2021, 22, 1843.

Grant projects

  • LTC17005 Multivalentní glykopolymery jako ligandy biologických struktur s lektinovou prezentací (2017-2020)
  • LTC18038 Multivalentní glyko-biomateriály s potenciálem v regenerační medicíně (2018-2021)
  • LTC19038 Cílené glyko-nanomateriály pro diagnostiku a terapii rakoviny (2019-2022)
  • 20-00215S Neutral oligosaccharides of human milk as ligands of galectins (2020-2022)
  • LTC20072 Library of engineered glycosidases for the preparation of bioactive glycans (2020-2023)
  • 22-00262S Advanced high-molecular-weight glycopolymers as potent inhibitors of galectin-induced pathological processes (2022-2024)
  • 22-00317S Galectin-3 as a target molecule for the reversal of vascular remodelling in hypoxic pulmonary hypertension (2022-2024)
  • CA2116 Identification of biological markers for prevention and translational medicine in pancreatic cancer (TRANSPAN)
  • CA21135 Modelling immunotherapy response and toxicity in cancer (IMMUNO-model)
  • CA20117 Converting molecular profiles of myeloid cells into biomarkers for inflammation and cancer (Mye-InfoBank)

Students & Teaching

  • 5 Ph.D. students (all currently studying)
  • 3 Master students (2 defended)
  • 16 Bachelor students (10 defended)

Faculty of Biomedical Engineering, Czech Technical University in Prague (FBMI ČVUT):

  • Environmentální bezpečnost, prezenční forma (17PBPENV)
  • Environmentální bezpečnost, kombinovaná forma (17KBP2ENB)
  • Obecné laboratorní dovednosti (17PBLOLD)

Participating also in:

  • Základy patologie, hygieny a epidemiologie (17PBBZPD)
  • Fundamentals of Pathophysiology & Diagnostic Methods (17AMBZPD)
  • Základy klinických oborů I (17KBIKO1)
  • Základy preklinické medicíny II (17PBIPM2)
  • Základy radiobiologie a možná zneužití radiačních a biologických agens (17PBPZRRBA)

Department of Biochemistry, Faculty of Science, Charles university (PřF UK)

  • Biochemie a biomedicína sacharidů (MC250P64)
  • Pokročilé praktikum II (MC250C08)

My core team

Organic synthesis: Mgr. David Vrbata

Biochemistry, molecular biology: Mgr. Pavlína Nekvasilová, Mgr. Michaela Hovorková, Mgr. Jakub Červený, MSc. Viktoria Heine

Biology: Ing. Miluše Vlachová, PhD.

Past team members (last 5 years): Ing. Tereza Strnadová Koukalová, Mgr. Tomáš Vašíček, Bc. Lieselotte Sedláková, Bc. Iveta Andreasová, Bc. Nikola Rychlá, Mgr. Veronika Libová, RNDr. Barbora Mikulová, BSc. Petr Brož, Ing. Dorota Konvalinková

What´s up

  • I am a Member of the Scientific and Organizational Committees of Biotech 2020, chairing the Section on Industrial Biotechnology 
  • I am Assistant Editor of the journal Folia Microbiologica (Springer)
  • I was awarded Invited lecture at 30th International Carbohydrate Symposium (Shanghai, July 2020)
  • We welcome students at all levels to join us for their Bachelor, Master or Dissertation Thesis

Snapshots from the life of our team

Last update: March 09, 2022