Scientific Program

Glyconanoprobes

synBIOcarb                                                                                                                         

TRAINING SCHOOL: ”ADVANCED TRAINING IN SYNTHESIS AND APPLICATIONS OF MULTIVALENT GLYCONANOMATERIALS”

Conference Chair: Vladimír Křen
Conference Secretary: Pavla Bojarová

Tuesday 29th March
COST TRAINING SCHOOL (Institute of Molecular Genetics, building F)
08.30 – 09.00 Registration
09.00 – 09.20 Welcome – M.C. Galan, B.W. Turnbull, V. Křen
09.20 – 11.00 SynBIOcarb ESR Individual Presentations, Chair: Dajana Kolanović
09.20 – 09.40 O-01 Laia Saltor Núñez (Leeds, UK) – Derivatization of hyaluronan for mucin-like structures in glycocalyx models (P-2)
09.40 – 10.00 O-02 Chunyue (Spring) Wang (Leeds, UK) – Superselective targeting of tumour cells
10.00 – 10.20 O-03 Lina Siukstaite (Freiburg, DE) – The two sweet sides of janus lectin drive crosslinking of liposomes to cancer cells and material uptake
10.20 – 10.40 O-04 Francesca Rosato (Freiburg, DE) – A clickable lectibody activates CD8+ cytotoxic T cells and induces cancer cell death (P-4)
10.40 – 11.00 O-05 Simona Notova (Grenoble, FR) – Punching holes in cancer cells: A pore-forming ß-trefoil lectin with specificity for the tumor-related glycosphingolipid Gb3 (P-7)
11.00 – 11.30 Coffee Break, poster installation (registration)
11.30 – 13.15 SynBIOcarb ESR Individual Presentations, Chair: Rajeev Pasupuleti
11.30 – 11.50 O-06 Dylan Jabeguero (Grenoble, FR) – Expansion of synthetic glycobiology tools: Altering the donor specificity of a glycosyltransferase (P-5)
11.50 – 12.10 O-07 Dajana Kolanović (Vienna, AT) – Directed surface immobilization of single-chain galectin-1 improves its ligand binding (P-16)
12.10 – 12.30 O-08 Rajeev Pasupuleti (Vienna, AT) – Lectin-antibody Stx1B-OKT3 induces T cell triggered immune response against CD77+ tumor cells (P-18)
12.30 – 12.50 O-09 Juvissan Aguedo (Bratislava, SK) – Application of MXenes for high enrichment of N-glycans and differentiation of sialic acid linkages (P-15)
12.50 – 13.10 O-10 Paras Kundalia (Bratislava, SK) – Lectin-based glycoprotein microarray: crucial parameters and application in cancer patients’ sera glycoprofiling (P-17)
13.15 – 14.30 Lunch
14.30 – 15.30 Hot Topics in glycobiology (tutorials), Chair: M. Carmen Galan
14.30 – 14.50 T-01 Hot Topic 1: Birte Svensson (Kgs. Lyngby, DK) – Rational enzyme design without structural knowledge: a sequence-based approach for efficient generation of tranglycosylases Virtual
14.50 – 15.10 T-02 Hot Topic 2: Rob Young  (Blue Burgundy Ltd., UK) – Some physical aspects of carbohydrate drug binding
15.10 – 15.30 T-03 Hot Topic 3: Rob Field (Manchester, UK) – Sequence, structure, function in glycospace 
15.30 – 18.00 Coffee Break, posters
16.00 – 17.00 External Advisory Board (EAB) ESR Tutorials
17.00 – 18.00 Poster Session
18.00 – 21.00 Conference Dinner – get together (Institute of Molecular Genetics, building F)
Wednesday 30th March
COST TRAINING SCHOOL (Institute of Microbiology, building A)
09.00 – 9.30 Registration (Institute of Microbiology, building A)
09.30 – 11.10 SynBIOcarb ESR Individual Presentation, Chair: Bruce Turnbull
09.30 – 09.50 O-11 Federica Vena (L’Orleans, FR) – Lectin array for quality control of recombinant biotherapeutic  glycoproteins
09.50 – 10.10 O-12 Natalia Danielewicz (Vienna, AT) – Process development for high yield fermentation of active recombinant lectins expressed in Escherichia coli (P-6)
10.10 – 10.30 O-13 Wenyue (Eva) Dai (Leeds, UK) – Assemble multiple CTB5 by coiled coil for GUV fusion Virtual
10.30 – 10.50 O-14 Spyridon Gatos (Lungby, DK) – Novel strategies and technologies for the development of tumor therapeutic antibodies (P-22) Virtual
10.50 – 11.10 O-15 Ilaria Mainero Rocca (Lungby, DK) – Glycoengineered Microbiome-based anti- infectives and novel-glycomic display systems (P-21) Virtual
11.10 – 12.10 Coffee Break, posters (Institute of Molecular Genetics, building F)
12.10 – 13.30 Hot Topics in multivalent glyconanomaterials, Chair: Viktoria Heine (Institute of Microbiology, building A)
12.10 – 12.30 T-04 Paul V. Murphy (Galway, IE) – Protecting group chemistry for synthetic glycoscience (tutorial)
12.30 – 12.50 T-05 Trinidad Velasco-Torrijos (Maynooth, IE) – Conjugation approaches for glycomaterials (tutorial)
12.50 – 13.10 O-16 K.F. Warfel (Evantston, US) – Cell-free protein synthesis: applications in synthetic glycobiology
13.10 – 13.30 O-17 Ralf Richter (Leeds, UK) – A novel sliding interaction between the extracellular matrix polysaccharide hyaluronan and the lymphatic vessel endothelial receptor LYVE-1 that regulates immune cell trafficking
13.30 – 14.30 Lunch (Institute of Molecular Genetics, building F)
14.30 – 15.30 COST TRAINING SCHOOL, Chair: Paul V. Murphy (Institute of Microbiology, building A)
14.30 – 14.50    T-06 M. Carmen Galan (Bristol, UK) – Novel multivalent synthetic platforms as potential theranostics (tutorial)
14.50 – 15.10    O-18 Manuel Gonzalez-Cuesta (Seville, ES) – sp2 – Iminosugar glycomimetics for OGA profiling
15.10 – 15.30    O-19 Carina Dey (Aachen, DE) – Characterization of galectin fusion proteins with glycoprotein affinity columns and  binding assays
15.30 – 16.30 Coffee Break, posters (Institute of Molecular Genetics, building F)
16.30 – 18.00 COST TRAINING SCHOOL, Chair: Trinidad Velasco-Torrijos (Institute of Microbiology, building A)
16.30 – 16.50 T-07 Miluše Vlachová (Prague, CZ) – Biological methods of assessment of galectin-glyconanomaterial interactions (tutorial)
16.50 – 17.10 O-20 Francesco Milanesi (Florence, IT) – Synthetic receptors for carbohydrates: an effective tool to interfere in carbohydrates recognition
17.10 – 17.30 O-21 Michaela Hovorková (Prague, CZ) – Preparation of high-affinity galectin ligands and their multivalent effect
17.30 – 17.50 O-22 Irene Herrera González (Seville, ES) – Synthesis of sp2-iminosugar-based mannobioside glycomimetics ligands targeting c-type lectin receptors (P-10)
  Free evening
Thursday 31th March
COST TRAINING SCHOOL & Workshop  (Institute of Molecular Genetics, building F)
09.00 – 9.30 Registration
09.30 – 10.40 COST TRAINING SCHOOL & Workshop sessions, Chair: Priyanka Sahariah
09.30 – 10.00 T-08 Pavla Bojarová (Prague, CZ) – Targeting galectins with high-affinity glycoconjugates – successes, challenges, and bottlenecks (tutorial)
10.00 – 10.20 O-23 Marcela Šišić (Zagreb, HR) – Functional glyconanomaterials based on bacterial peptidoglycan for targeted drug delivery and surface recognition studies
10.20 – 10.40 O-29 Simon Johnson (Bristol, UK) – Synthesis of multivalent mucin-type O-glycan analogues for use as diagnostic probe
10.40 – 11.10 Coffee Break, posters
11.10 – 13.00 COST TRAINING SCHOOL & Workshop sessions, Chair: Zdeněk Wimmer
11.10 – 11.30 O-25 David Vrbata (Prague, CZ) – Multivalent glycomimetics as inhibitors of galectin-3
11.30 – 11.50 O-26 Adamantia Apostolopoulou (Athens, GR) – In vivo tracking of radiolabeled nanostructures
11.50 – 12.10 O-27 Maria Psarrou (Crete, GR) – Polysaccharide-functionalized iron oxide nanoparticles for mRNA delivery
12.10 – 12.30 O-28 Joana Gonçalves (Aveiro, PT) – Development of magnetic composites for magnetic hyperthermia therapy and bone regeneration
12.30   Conference dinner information
12.30 – 14.00 Lunch (posters to be removed)
14.00 – 17.00 Networking COST Working group 1
18.00 – 21.00 Conference Dinner
Friday 1st April
COST Workshop (Institute of Microbiology, building A)
09.00 – 09.30 Registration
09.30 – 12.00 COST sessions, Chair: Kateřina Valentová
09.30 – 09.50 T-09 Priyanka Sahariah (Reykjavik, IS) – Chitosan peptide conjugates against bacterial biofilms (tutorial)
09.50 – 10.10 T-10 Vladimír Křen (Prague, CZ) – Enzymatic synthesis of glycoconjugates with glycosidases: Diglycosidase rutinosidase (tutorial)
10.10 – 10.30 O-30 Viktoria Heine (Prague, CZ) – Poly-LacNAc-decorated neo-glycoproteins and their interaction with tandem-repeat galectins
10.30 – 10.50 O-31 Ana Barra (Aveiro, PT) – Development of biopolymer-based electrically conductive  biocomposites
10.50 – 11.10 O-33 Jack Schofield (Galway, IE) – Fucosylation inhibition ameliorates inflammatory glycosylation & metabolism in corneal fibroblasts (P-20)
11.10 – 11.30 free slot
11.30 – 12.00                    Coffee Break, packed lunch boxes and departure for travel                                                                                                                                                                                                                                          

 

List of Posters

  1. Siukstaite (Freiburg, D): The two sweet sides of janus lectin drive crosslinking of liposomes to cancer cells and material uptake (O-3)
  2. (Spring) Wang & L.S. Núñez (Leeds, UK): Derivatization of hyaluronan and their applications (O-1)
  3. F. Warfel (Evantston, US) A low-cost, thermostable, cell-free protein synthesis platform for on-demand production of glycoconjugate vaccines
  4. Rosato (Freiburg, D) A clickable lectibody activates CD8+ cytotoxic T cells and induces cancer cell death (O-4)
  5. Jabeguero (Grenoble, F) Expansion of synthetic glycobiology tools: Altering the donor specificity of a glycosyltransferase (O-6)
  6. Danielewicz (Vienna, A) Process development for high yield fermentation of active recombinant lectins expressed in Escherichia coli (O-12)
  7. Notova (Grenoble, F) Janus lectins and neo-lectins: engineering of new scaffolds and topology (O-5)
  8. M. Saladino (Stockholm, S) Single step synthesis of sugar ligand coated superparamagnetic iron oxide nanoparticles with antibacterial properties
  9. Fitzgerald (Gatway, IE) Outcome of docking of some simple sialic acid derivatives to influenza hemagglutinin and synthesis of glycoclusters based on tetraphenylethylene scaffold
  10. Herrera González (Seville, E) Synthesis of sp2-iminosugar-based mannobioside glycomimetics ligands targeting c-type lectin receptors (O-22)
  11. Meszáros (Prague, CZ) Mutant hypertransglycosylating GH20 β-N-hexosaminidases for the synthesis of bioactive chitooligomers
  12. Brodsky (Prague, CZ) Rutinosidase and its application in the synthesis of glycoconjugates
  13. Červený (Prague, CZ) Biolayer interferometry – a great tool for decoding the kinetic of lectin-carbohydrate interaction
  14. Konvalinková (Prague, CZ) Glycocalix[4]arenes and their affinity to a galectin library
  15. Aguedo & F. Vena (Bratislava, SK; Orleans, FR) Rapid and high throughput methods for discrimination of sialic acids linkages in glycoproteins
  16. Kolanović (Graz, AT) Directed surface immobilization of single-chain galectin-1 improves its ligand binding (O-7)
  17. Kundalia (Bratislava, SK) Lectin-based glycoprotein microarray: crucial parameters and application in cancer patients’ sera glycoprofiling (O-10)
  18. Pasupuleti (Vienna, AT) Lectin-antibody Stx1B-OKT3 induces T cell triggered immune response against CD77+ tumor cells (O-8)
  19. Wenyue Dai (Leeds, UK) Exploring structure-activity relationships of defined streptavidin-bacterial toxin assemblies that induce membrane crosslinking and fusion
  20. Schofield (Galway, IE) Fucosylation inhibition ameliorates inflammatory glycosylation & metabolism in corneal fibroblasts (O-33)
  21. Rocca (Lyngby, DK) Glycoengineered Microbiome-based anti-infectives and novel-glycomic display systems (O-15)
  22. Gatos (Lyngby, DK) Novel strategies and technologies for the development of tumor therapeutic antibodies
  23. Illenseer Katharina (Konstanz, DE) Synthesis of multivalent AB5 toxin inhibitors

 

Program pdf version

Abstracts